Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   J5X95_RS03005 Genome accession   NZ_CP072120
Coordinates   591854..594211 (-) Length   785 a.a.
NCBI ID   WP_012118134.1    Uniprot ID   A7Z7E7
Organism   Bacillus amyloliquefaciens strain GKT04     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 586854..599211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X95_RS02980 (J5X95_02980) - 587357..588136 (-) 780 WP_032866568.1 enoyl-CoA hydratase -
  J5X95_RS02985 (J5X95_02985) - 588150..588734 (-) 585 WP_007408119.1 TetR/AcrR family transcriptional regulator -
  J5X95_RS02990 (J5X95_02990) - 588823..590511 (-) 1689 WP_061046575.1 AMP-binding protein -
  J5X95_RS02995 (J5X95_02995) - 590617..591294 (+) 678 WP_007408117.1 DUF2711 family protein -
  J5X95_RS03000 (J5X95_03000) - 591433..591837 (-) 405 WP_003152541.1 DUF350 domain-containing protein -
  J5X95_RS03005 (J5X95_03005) mutS/mutS2 591854..594211 (-) 2358 WP_012118134.1 endonuclease MutS2 Machinery gene
  J5X95_RS03010 (J5X95_03010) polX 594232..595944 (-) 1713 WP_007408115.1 DNA polymerase/3'-5' exonuclease PolX -
  J5X95_RS03015 (J5X95_03015) - 596050..596583 (-) 534 WP_007612958.1 CvpA family protein -
  J5X95_RS03020 (J5X95_03020) zapA 596591..596848 (-) 258 WP_007408114.1 cell division protein ZapA -
  J5X95_RS03025 (J5X95_03025) rnhC 596982..597923 (+) 942 WP_007408113.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87233.51 Da        Isoelectric Point: 6.3216

>NTDB_id=480712 J5X95_RS03005 WP_012118134.1 591854..594211(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain GKT04]
MQQKVLSSLEFHKVKEQITAHAASSLGREKLLQLKPLTDLSDIQKQLDEVEEASAVMRLRGHAPFGGLTDIRSALRRAEI
GSVLTPAEFTELSGLLYAVKQMKHFISQMTEDGVGIPLIQAHAEELITLGDLEREINSCIDDHGEVLDHASPALRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRMLTEHTAEHTQEIAQDVEVLQTLDFIFAKARYAKAMKATKPLMNGDGFIRLKKARHPLLPQDQVVAN
DIELGGDYSTIVITGPNTGGKTVTLKTLGLLTIMAQAGLHIPADEGSEAAVFDNVFADIGDEQSIEQSLSTFSSHMVNIV
NILKDVSENSLVLFDELGAGTDPQEGAALAMSILDEVHRTNARVLATTHYPELKAYGYNRQGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISRRLGLPEHIIGQAKSEMTAEHNEVDLMIASLEKSKKRADEELSETESIRKEAEKLHKDLQQQIIE
LNAQKDKMMEEAEQKAAEKLEAAANEAEQIIRELRSIKQEHRSFKEHELIDAKKRLGDAMPAFEKSKQPERKTEKKRELK
PGDEVKVLTFGQKGALLEKTGEKEWNVQIGILKMKVKEKDLEFLKSAPEPKKEKAITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGHPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTIVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=480712 J5X95_RS03005 WP_012118134.1 591854..594211(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain GKT04]
GTGCAGCAAAAAGTGTTATCATCACTTGAATTTCATAAAGTAAAAGAACAGATTACCGCACATGCCGCCTCATCGCTCGG
CCGGGAGAAGCTTCTGCAGCTGAAACCTTTGACGGATCTCTCAGACATCCAAAAACAGCTGGATGAAGTCGAAGAAGCAT
CCGCGGTCATGCGCTTGAGAGGCCATGCTCCGTTCGGCGGTCTGACCGACATAAGATCCGCTTTACGGCGGGCGGAAATC
GGCAGTGTGCTGACGCCGGCGGAATTCACCGAATTGTCCGGGCTGCTGTATGCAGTTAAACAAATGAAGCACTTTATCAG
CCAAATGACAGAAGACGGCGTCGGCATTCCGCTGATACAGGCCCATGCAGAAGAACTGATTACATTGGGCGATTTGGAAA
GGGAGATTAACTCCTGCATTGATGACCATGGTGAAGTGCTTGATCATGCGTCTCCCGCTTTAAGGGGAATCCGCACGCAG
CTCAGGACGCTTGAATCAAGAGTCAGAGACCGGCTTGAATCGATGCTGCGTTCATCTTCCGCTTCTAAAATGCTGTCTGA
CACCATCGTTACGATTCGGAATGACCGCTTTGTCATCCCGGTCAAACAGGAATACAGATCAAGCTACGGCGGAATTGTCC
ACGATACATCTTCATCCGGTGCGACACTGTTTATTGAACCGCAGGCGATCGTAGATATGAACAACTCGCTTCAGCAGGCG
AAAGTAAAAGAAAAACAGGAAATCGAACGGATTCTCCGGATGCTGACGGAGCATACGGCTGAACACACACAAGAAATCGC
TCAAGATGTGGAAGTGCTGCAGACACTGGATTTCATTTTCGCCAAAGCGAGATACGCAAAAGCAATGAAGGCGACAAAAC
CACTCATGAACGGAGACGGTTTTATCCGGCTGAAAAAAGCGCGCCATCCGCTGCTGCCCCAAGATCAAGTCGTGGCCAAC
GATATTGAACTGGGCGGAGATTACTCAACGATCGTTATCACGGGACCGAATACGGGCGGTAAAACCGTTACGTTAAAAAC
ACTCGGGCTGCTGACGATAATGGCGCAGGCAGGATTGCACATACCGGCTGATGAGGGATCGGAAGCAGCAGTATTTGACA
ATGTGTTTGCCGATATCGGCGATGAACAGTCGATCGAACAAAGTCTGAGTACGTTCTCATCCCACATGGTCAATATCGTC
AATATTTTAAAAGACGTGTCTGAAAACAGTCTTGTGCTGTTTGATGAACTCGGTGCCGGAACAGATCCGCAGGAAGGAGC
GGCGCTTGCCATGAGCATCCTTGATGAAGTTCACCGGACAAACGCCAGAGTGCTTGCGACAACCCATTATCCGGAACTGA
AAGCATACGGATATAACAGACAAGGCGTCATGAATGCCAGCGTTGAATTTGACATTGAAACGCTTTCGCCTACCTATAAG
CTTCTGATCGGAGTGCCTGGCCGAAGCAACGCATTTGAAATCTCAAGACGCCTTGGGCTTCCGGAGCATATCATCGGCCA
GGCGAAGTCAGAAATGACCGCCGAACATAACGAAGTCGATCTGATGATCGCATCGCTTGAAAAAAGCAAAAAAAGAGCGG
ATGAAGAGCTTTCTGAAACCGAATCAATCAGAAAAGAAGCAGAAAAACTGCACAAAGATCTTCAGCAGCAAATCATTGAG
CTGAACGCCCAAAAAGATAAAATGATGGAAGAAGCCGAGCAGAAAGCTGCGGAAAAACTGGAAGCCGCCGCAAATGAAGC
CGAACAGATTATCCGCGAGCTCAGGTCCATCAAGCAAGAACACAGATCCTTTAAGGAACACGAGCTGATTGACGCCAAAA
AACGTCTCGGAGACGCGATGCCGGCTTTTGAAAAATCAAAGCAGCCGGAAAGAAAGACAGAGAAAAAACGCGAGCTCAAG
CCGGGCGACGAAGTAAAGGTGCTCACATTCGGACAAAAAGGGGCGCTGCTTGAGAAAACCGGCGAGAAAGAATGGAACGT
CCAAATCGGCATCCTCAAAATGAAGGTGAAGGAAAAAGATCTTGAGTTTCTCAAATCGGCTCCTGAACCGAAAAAAGAAA
AAGCAATCACCGCCGTAAAAGGGAAGGATTATCACGTATCCCTTGAACTTGACCTCAGGGGAGAGCGTTACGAAAACGCC
CTCAGCCGCGTCGAAAAATATTTAGATGACGCGGTGCTTGCCGGCCATCCGAGAGTGTCCATCATTCACGGAAAAGGCAC
GGGCGCCCTCCGAAAAGGGGTGCAGGATCTTCTGAAAAACCACCGCAGCGTGAAAAGCTCGCGGTTCGGGGAAGCTGGAG
AAGGAGGATCAGGCGTAACGATTGTCGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

Smr domain (Smr)

(710-785)

MutS domain V (MutS_V)

(331-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z7E7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

87.516

100

0.875