Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   J5V74_RS01455 Genome accession   NZ_CP071947
Coordinates   317772..318197 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2020HL-00861     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 312772..323197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5V74_RS01440 (J5V74_01440) pilX 313336..313923 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  J5V74_RS01445 (J5V74_01445) pilY1 313935..317426 (+) 3492 WP_023086645.1 type 4a pilus biogenesis protein PilY1 -
  J5V74_RS01450 (J5V74_01450) pilY2 317428..317775 (+) 348 WP_012614546.1 type 4a fimbrial biogenesis protein PilY2 -
  J5V74_RS01455 (J5V74_01455) comF 317772..318197 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  J5V74_RS01460 (J5V74_01460) ispH 318244..319188 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  J5V74_RS01465 (J5V74_01465) fkpB 319274..319714 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  J5V74_RS01470 (J5V74_01470) lspA 319707..320216 (-) 510 WP_003110420.1 signal peptidase II -
  J5V74_RS01475 (J5V74_01475) ileS 320209..323040 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=479735 J5V74_RS01455 WP_003094721.1 317772..318197(+) (comF) [Pseudomonas aeruginosa strain 2020HL-00861]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=479735 J5V74_RS01455 WP_003094721.1 317772..318197(+) (comF) [Pseudomonas aeruginosa strain 2020HL-00861]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383