Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   NMV_RS07475 Genome accession   NC_017501
Coordinates   1474788..1475387 (-) Length   199 a.a.
NCBI ID   WP_014574101.1    Uniprot ID   A0A9K2PS66
Organism   Neisseria meningitidis 8013     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1443769..1479361 1474788..1475387 within 0


Gene organization within MGE regions


Location: 1443769..1479361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMV_RS07325 (NMV_1479) - 1443769..1444137 (+) 369 WP_002217436.1 type II toxin-antitoxin system death-on-curing family toxin -
  NMV_RS07330 (NMV_1481) - 1444267..1444503 (+) 237 WP_002217439.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  NMV_RS07335 (NMV_1482) - 1444505..1444852 (+) 348 WP_002217440.1 type II toxin-antitoxin system PemK/MazF family toxin -
  NMV_RS07340 (NMV_1483) - 1444905..1445414 (-) 510 WP_002245556.1 hypothetical protein -
  NMV_RS07345 (NMV_1485) - 1445426..1445899 (-) 474 WP_014574085.1 D-Ala-D-Ala carboxypeptidase family metallohydrolase -
  NMV_RS07350 (NMV_1486) - 1446047..1446427 (+) 381 WP_002238475.1 type II toxin-antitoxin system PemK/MazF family toxin -
  NMV_RS15580 (NMV_1487) - 1446632..1446781 (-) 150 WP_014574086.1 hypothetical protein -
  NMV_RS07360 (NMV_1488) - 1446784..1447458 (-) 675 WP_014574087.1 hypothetical protein -
  NMV_RS07365 (NMV_1489) - 1447455..1447862 (-) 408 WP_014574088.1 hypothetical protein -
  NMV_RS07370 (NMV_1490) - 1447946..1448419 (-) 474 WP_014574089.1 hypothetical protein -
  NMV_RS07375 (NMV_1491) - 1448477..1449772 (-) 1296 WP_002256560.1 DUF4043 family protein -
  NMV_RS15910 (NMV_1492) - 1449793..1450704 (-) 912 WP_014574090.1 hypothetical protein -
  NMV_RS07385 (NMV_1493) - 1450789..1451982 (-) 1194 WP_014574091.1 hypothetical protein -
  NMV_RS07390 (NMV_1494) - 1451970..1455176 (-) 3207 WP_014574092.1 hypothetical protein -
  NMV_RS07395 (NMV_1495) - 1455188..1455580 (-) 393 WP_014574093.1 hypothetical protein -
  NMV_RS07400 (NMV_1496) - 1455580..1456479 (-) 900 WP_014574094.1 hypothetical protein -
  NMV_RS07405 (NMV_1497) - 1456472..1456897 (-) 426 WP_021439711.1 hypothetical protein -
  NMV_RS07410 (NMV_1499) - 1456909..1457316 (-) 408 WP_002238463.1 hypothetical protein -
  NMV_RS13070 (NMV_1500) - 1457419..1464810 (-) 7392 WP_231844692.1 PLxRFG domain-containing protein -
  NMV_RS15915 (NMV_1501) - 1465802..1466218 (-) 417 WP_014574097.1 hypothetical protein -
  NMV_RS07430 (NMV_1502) - 1466215..1467411 (-) 1197 WP_002256553.1 hypothetical protein -
  NMV_RS07435 (NMV_1503) - 1467675..1468871 (-) 1197 WP_014574098.1 tyrosine-type recombinase/integrase -
  NMV_RS07450 (NMV_1505) yaaA 1469403..1470182 (-) 780 WP_002238459.1 peroxide stress protein YaaA -
  NMV_RS07455 (NMV_1506) dapC 1470345..1471532 (-) 1188 WP_014574099.1 succinyldiaminopimelate transaminase -
  NMV_RS07460 (NMV_1507) dut 1471609..1472061 (-) 453 WP_002222651.1 dUTP diphosphatase -
  NMV_RS07465 (NMV_1508) - 1472207..1472635 (+) 429 Protein_1386 AzlC family ABC transporter permease -
  NMV_RS07470 (NMV_1509) pilX 1474310..1474783 (-) 474 WP_173277181.1 PilX family type IV pilin Machinery gene
  NMV_RS07475 (NMV_1510) pilK 1474788..1475387 (-) 600 WP_014574101.1 pilus assembly PilX family protein Machinery gene
  NMV_RS07480 (NMV_1511) pilJ 1475366..1476331 (-) 966 WP_014574102.1 PilW family protein Machinery gene
  NMV_RS07485 (NMV_1512) pilV 1476328..1476948 (-) 621 WP_014574103.1 type IV pilus modification protein PilV Machinery gene
  NMV_RS07490 (NMV_1513) pilH 1476981..1477646 (-) 666 WP_014574104.1 GspH/FimT family pseudopilin Machinery gene
  NMV_RS07495 (NMV_1514) dnaB 1477955..1479361 (-) 1407 WP_002219448.1 replicative DNA helicase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 21971.85 Da        Isoelectric Point: 5.0957

>NTDB_id=47745 NMV_RS07475 WP_014574101.1 1474788..1475387(-) (pilK) [Neisseria meningitidis 8013]
MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGELQVLDLEYDT
DSKVTFSENCGEGLCTAVNVRTNTNNDNEEAFDNIVVQGKPTVEAVKRFCPANSTDLCIDKKGMEYKKGTRSVSKPPRYI
IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE

Nucleotide


Download         Length: 600 bp        

>NTDB_id=47745 NMV_RS07475 WP_014574101.1 1474788..1475387(-) (pilK) [Neisseria meningitidis 8013]
ATGCGCAAACAGAACACTTTGACGGGAATCCCGACTTCTGACGGACAGAGGGGGTTTGCACTGTTTATCGTGCTGATGGT
GATGATCGTCGTGGCTTTTTTGGTTGTAACTGCCGCGCAGTCTTACAATACCGAGCAGCGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTGGCCGAGGCGGCTTTGCGGGAAGGCGAACTTCAGGTTTTGGATTTGGAATATGATACG
GACAGTAAGGTTACATTTAGCGAAAACTGTGGAGAAGGTCTGTGTACCGCAGTGAATGTGCGGACAAATACAAATAATGA
TAATGAAGAGGCTTTTGACAATATCGTGGTGCAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTTTTGCCCTGCAAATT
CTACCGACCTGTGCATTGACAAGAAAGGGATGGAATATAAGAAAGGCACGAGAAGCGTCAGCAAACCACCGCGCTATATT
ATCGAATATTTGGGCGTGAAGAACGGAGAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGCCAATACCGT
GGTCGTCCTTCAATCTTATGTAAGCAATAATGATGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilX C-term (PilX)

(92-194)

PilX N-terminal (PilX_N)

(17-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

85.714

100

0.874

Multiple sequence alignment