Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   J1F19_RS12060 Genome accession   NZ_CP071439
Coordinates   2513902..2514666 (+) Length   254 a.a.
NCBI ID   WP_001136250.1    Uniprot ID   -
Organism   Escherichia coli strain EcPNK004     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2508902..2519666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1F19_RS12040 (J1F19_12040) acpT 2509856..2510443 (+) 588 WP_000285781.1 4'-phosphopantetheinyl transferase AcpT -
  J1F19_RS12045 (J1F19_12045) nikA 2510554..2512128 (+) 1575 WP_000953368.1 nickel ABC transporter substrate-binding protein -
  J1F19_RS12050 (J1F19_12050) nikB 2512128..2513072 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  J1F19_RS12055 (J1F19_12055) nikC 2513069..2513902 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  J1F19_RS12060 (J1F19_12060) amiE 2513902..2514666 (+) 765 WP_001136250.1 nickel import ATP-binding protein NikD Regulator
  J1F19_RS12065 (J1F19_12065) nikE 2514663..2515469 (+) 807 WP_001586378.1 nickel import ATP-binding protein NikE -
  J1F19_RS12070 (J1F19_12070) nikR 2515475..2515876 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  J1F19_RS12075 (J1F19_12075) - 2516075..2516821 (+) 747 WP_001329803.1 GntR family transcriptional regulator -
  J1F19_RS12080 (J1F19_12080) - 2516846..2517319 (+) 474 WP_001306344.1 PTS sugar transporter subunit IIA -
  J1F19_RS12085 (J1F19_12085) - 2517316..2517597 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  J1F19_RS12090 (J1F19_12090) - 2517674..2519032 (+) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=476925 J1F19_RS12060 WP_001136250.1 2513902..2514666(+) (amiE) [Escherichia coli strain EcPNK004]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGSSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=476925 J1F19_RS12060 WP_001136250.1 2513902..2514666(+) (amiE) [Escherichia coli strain EcPNK004]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAACCGCTGGTGCACGGCGTATCGTTAACTCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCAGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCCGCACTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCTTTAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398