Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   H7F26_RS12310 Genome accession   NZ_CP060192
Coordinates   2414016..2417516 (+) Length   1166 a.a.
NCBI ID   WP_185855183.1    Uniprot ID   A0AAP9UNE8
Organism   Bacillus sp. PAMC28748     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2409016..2422516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F26_RS12285 (H7F26_12285) - 2409675..2411042 (-) 1368 WP_017474228.1 cell wall metabolism sensor histidine kinase WalK -
  H7F26_RS12290 (H7F26_12290) - 2411039..2411680 (-) 642 WP_044051832.1 response regulator transcription factor -
  H7F26_RS12295 (H7F26_12295) - 2411803..2412243 (-) 441 WP_009328902.1 ferritin-like domain-containing protein -
  H7F26_RS12300 (H7F26_12300) modA 2412447..2413241 (+) 795 WP_003180424.1 molybdate ABC transporter substrate-binding protein -
  H7F26_RS12305 (H7F26_12305) modB 2413250..2413906 (+) 657 WP_003180426.1 molybdate ABC transporter permease subunit -
  H7F26_RS12310 (H7F26_12310) addB 2414016..2417516 (+) 3501 WP_185855183.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  H7F26_RS12315 (H7F26_12315) addA 2417503..2421195 (+) 3693 WP_003180429.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  H7F26_RS12320 (H7F26_12320) sbcD 2421261..2422442 (+) 1182 WP_003180431.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134486.54 Da        Isoelectric Point: 5.5705

>NTDB_id=474429 H7F26_RS12310 WP_185855183.1 2414016..2417516(+) (addB) [Bacillus sp. PAMC28748]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLQPESIRDMIEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYADEIRNAAVYVDGFHQFTPQEMNVLEQLMVHAEEITFSLTADKP
FTANSPNDLHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHRHHPELQHLERYFDERPAKPYPGQTESLRIMQASNR
RTEIEGIAREIHSLIRQGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYASLDEDFAKTDKEIETENMLNELKGW
IVPPIHRLQKRLKKAETVREMAEAVYLYLEEADVPMKLEQERRLAEESGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDDDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDGEEKMLIHEPEQLDDEAQLEFLVNKG
AALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQMFHSALKLISDRLHELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMKASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNMISAGLKKDGSLRSDSDVIAEEDFHVLRRHIRRTFQKAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDNVILERLKKEDETDGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=474429 H7F26_RS12310 WP_185855183.1 2414016..2417516(+) (addB) [Bacillus sp. PAMC28748]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCCGATTTGGGCGGAACTATCCGCGCCCAAGTGTACAGTTTTTCAAGGCTTGCCTGGCGAGTGCTCCAGCATACCGGA
GGCATGAATCGGCCGTTTTTGACGGGGACGGGCATTCAGATGCTGCTGCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTTAAACGGCATT
GTCTGCAGCCTGAAAGCATCCGGGACATGATAGAGGGGACGGGCAAATCGGAATACGAAGATGAACGGATCCTTTCCGAT
AAATTGCACGACTTATATATTTTATACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAGGATTATTT
GACGCTTTTGGCCGAGCAGATTCCGTATGCGGATGAGATTAGAAACGCTGCCGTTTACGTGGACGGATTTCACCAATTTA
CGCCACAGGAGATGAACGTGCTGGAACAGCTGATGGTACATGCCGAAGAGATCACGTTTTCGCTGACGGCGGATAAGCCG
TTTACGGCAAACAGCCCGAATGATCTCCATCTGTTCAGGATGACCGGGAAGGCGTACTATGACCTTTACCAAAAGGCGAA
GGAACTCGGCCTTGATCCGTCTGAAGTGCGGCTGGAAGAAACGAAACGGCACCGGCATCATCCGGAGCTTCAGCATCTTG
AACGCTATTTTGATGAACGTCCTGCAAAACCGTATCCCGGACAGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATCCGCCAGGGCCGGTTCCGGCTTAGGGATATTGCTGT
CATCGCCCGCAATGTTGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGCGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTCATTGAGCTGATCCGTTCGACGCTTGATATCATTAAAGGAAACTGGCGGTAT
GAAGCGGTGTTCCGCTGTGTCAAAACGGAACTTTTATTCCCGGAAGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCGTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGGT
ACGCTTCACTGGATGAGGATTTTGCCAAGACAGACAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCACCGATCCACAGGCTGCAGAAGCGCTTGAAAAAAGCTGAAACCGTCAGGGAAATGGCCGAAGCCGTTTACCT
TTATCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTGGCCGAAGAAAGCGGTAGGATTGCAGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATA
TCGTTTGCTTTATTTCAGCAAATGATTGAAACCGGCTTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCTCTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGCACATTTCTCATCGGTGTAAACGACGGTATAC
TTCCGGCGAGGCCTGCGGACGACGGCGTGCTTTCAGACGATGACCGGGAGTGGCTGAAACGAAACGGCGCACAGCTGGCT
GCGACGGGTCGAGAGCAGCTCTTGGATGAGAATTTTCTGATCTACATGACGCTTTCCAGCCCGTCTGAAAAGCTCTATGT
CTCCTATCCGATCGCAGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTAGTGAAACGGCTGAATGAGCTGTTCCCGG
ACGGCGAAGAGAAGATGCTGATTCATGAACCCGAGCAGCTGGACGACGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
GCCGCCTTGAGCCATTTGGCAGGGCAGCTCGGCATTTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAGCCTGACCGCATCTTTTCTCAAAATATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACTTGAACCGGCATGTGAGCCGCGATTTATATGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAAGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGTTTAAAGCTGAAGGAACGTCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGATGTTCCACTCGGCGCTGAAGCTGATTTCAGATCGGCTTCACGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAATGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTCGCGCCAAAGCTGCAAAAAGAGATTTTGCTCAGTTCGAAC
CGTCATCATTACGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGCGAGCATGCAAAAGCAAG
CGGGTTTGCCCCCGTCGGCATCGAGCTCGGATTCGGGGGGAAAGGTCCGCTTCCTCCAATGCGTTTTACCTTGAAGAACG
GCTGCACAATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGCTGCTGCTTAGGATTGTC
GACTATAAATCAAGCGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGCCTCGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACACATTCAACCGATTGGCTCGGCATGAAGGCATCCCCTGCAGGCGTGCTTTATTTTCATGTGCACGATC
CGATGATCCAGGCTTCGGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTG
CTCTTGGGAGACCAGGAAGCGGTCAAGCTGATGGATACCACGCTGGAACAAGGCAGGTCGAACATGATCAGCGCAGGCTT
GAAAAAAGACGGCTCGCTCCGTTCGGATTCTGACGTTATTGCAGAAGAGGATTTTCATGTATTAAGGCGCCATATCCGCC
GGACATTTCAGAAGGCCGGCGAGGAGATCACAGACGGTAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCGTACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGGATATTAAAACCCGA
AAAAGACAACGTGATTTTGGAGCGGCTGAAAAAGGAGGATGAGACAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

73.82

99.914

0.738