Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   R2866_RS01050 Genome accession   NC_017451
Coordinates   207613..208155 (-) Length   180 a.a.
NCBI ID   WP_005666612.1    Uniprot ID   -
Organism   Haemophilus influenzae R2866     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 202613..213155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2866_RS01020 (R2866_0203) fdx 203016..203357 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  R2866_RS01025 (R2866_0204) iscX 203357..203551 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  R2866_RS01030 (R2866_0205) - 203602..204216 (-) 615 WP_005666604.1 YfgM family protein -
  R2866_RS01035 (R2866_0206) hisS 204234..205505 (-) 1272 WP_005666606.1 histidine--tRNA ligase -
  R2866_RS01040 (R2866_0207) ispG 205515..206621 (-) 1107 WP_005666608.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  R2866_RS01045 (R2866_0208) - 206630..207541 (-) 912 WP_005666610.1 helix-turn-helix domain-containing protein -
  R2866_RS01050 (R2866_0209) pilF/pilF2 207613..208155 (-) 543 WP_005666612.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  R2866_RS01055 (R2866_0210) - 208256..209407 (-) 1152 WP_005666614.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  R2866_RS01060 (R2866_0211) - 209654..210532 (+) 879 WP_005649130.1 D-alanyl-D-alanine carboxypeptidase family protein -
  R2866_RS01065 (R2866_0212) - 210678..211814 (-) 1137 WP_005666616.1 RNA-guided endonuclease InsQ/TnpB family protein -
  R2866_RS01070 (R2866_0213) - 212189..213070 (+) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20807.49 Da        Isoelectric Point: 8.6167

>NTDB_id=47254 R2866_RS01050 WP_005666612.1 207613..208155(-) (pilF/pilF2) [Haemophilus influenzae R2866]
MQTISKQLSAVIFPFIFSACVSQSTIQDFNQQSAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQ
QQGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQ
TLEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=47254 R2866_RS01050 WP_005666612.1 207613..208155(-) (pilF/pilF2) [Haemophilus influenzae R2866]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCCCAATCAACTATTCA
AGACTTCAATCAACAATCAGCAGCGAAAGCTCGCGTAGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGG
CTAAAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAA
CAACAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAA
TAATTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGA
ATTATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAA
ACATTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

93.889

100

0.939
  pilF Glaesserella parasuis strain SC1401

48.045

99.444

0.478

Multiple sequence alignment