Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   H3M14_RS06170 Genome accession   NZ_CP059697
Coordinates   1217635..1218342 (+) Length   235 a.a.
NCBI ID   WP_226474265.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain CBA3635     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1212635..1223342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3M14_RS06140 (H3M14_06140) - 1212844..1214031 (+) 1188 WP_025015730.1 FtsW/RodA/SpoVE family cell cycle protein -
  H3M14_RS06145 (H3M14_06145) - 1214042..1215130 (+) 1089 WP_025015731.1 CAP-associated domain-containing protein -
  H3M14_RS06150 (H3M14_06150) - 1215154..1215444 (+) 291 WP_016265218.1 YlbG family protein -
  H3M14_RS06155 (H3M14_06155) rsmD 1215449..1216003 (+) 555 WP_016265217.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  H3M14_RS06160 (H3M14_06160) coaD 1216016..1216513 (+) 498 WP_025015732.1 pantetheine-phosphate adenylyltransferase -
  H3M14_RS06165 (H3M14_06165) - 1216515..1217561 (+) 1047 WP_035145546.1 SepM family pheromone-processing serine protease -
  H3M14_RS06170 (H3M14_06170) comEA 1217635..1218342 (+) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  H3M14_RS06175 (H3M14_06175) - 1218400..1218888 (+) 489 WP_011374770.1 ComE operon protein 2 -
  H3M14_RS06180 (H3M14_06180) comEC 1219086..1221152 (+) 2067 WP_231953244.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  H3M14_RS06185 (H3M14_06185) holA 1221149..1222177 (+) 1029 WP_025015735.1 DNA polymerase III subunit delta -
  H3M14_RS06190 (H3M14_06190) rpsT 1222245..1222499 (-) 255 WP_011374767.1 30S ribosomal protein S20 -
  H3M14_RS06195 (H3M14_06195) rpsO 1222778..1223047 (+) 270 WP_011374766.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26122.75 Da        Isoelectric Point: 9.7683

>NTDB_id=471403 H3M14_RS06170 WP_226474265.1 1217635..1218342(+) (comEA) [Latilactobacillus sakei strain CBA3635]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=471403 H3M14_RS06170 WP_226474265.1 1217635..1218342(+) (comEA) [Latilactobacillus sakei strain CBA3635]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

97.021

100

0.97