Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   H1W83_RS24425 Genome accession   NZ_CP059457
Coordinates   4658627..4660969 (-) Length   780 a.a.
NCBI ID   WP_237892996.1    Uniprot ID   -
Organism   Priestia megaterium strain 2020WEIHUA_L     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4653627..4665969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W83_RS24400 (H1W83_24245) - 4654020..4654196 (-) 177 WP_237892992.1 RNA polymerase subunit sigma-70 -
  H1W83_RS24405 (H1W83_24250) - 4654363..4655247 (+) 885 WP_237892994.1 N-acetylmuramoyl-L-alanine amidase -
  H1W83_RS24410 (H1W83_24255) dtd 4655238..4655699 (-) 462 WP_016765630.1 D-aminoacyl-tRNA deacylase -
  H1W83_RS24415 (H1W83_24260) - 4655715..4657913 (-) 2199 WP_013059308.1 RelA/SpoT family protein -
  H1W83_RS24420 (H1W83_24265) - 4658099..4658611 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  H1W83_RS24425 (H1W83_24270) recJ 4658627..4660969 (-) 2343 WP_237892996.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  H1W83_RS24430 (H1W83_24275) secDF 4661110..4663347 (-) 2238 WP_016765632.1 protein translocase subunit SecDF -
  H1W83_RS24435 (H1W83_24280) - 4663485..4663778 (-) 294 WP_016765633.1 post-transcriptional regulator -
  H1W83_RS24440 (H1W83_24285) spoVB 4663843..4665453 (+) 1611 WP_080743079.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87983.65 Da        Isoelectric Point: 4.7478

>NTDB_id=469732 H1W83_RS24425 WP_237892996.1 4658627..4660969(-) (recJ) [Priestia megaterium strain 2020WEIHUA_L]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGRTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPILPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKV
RQQIVNDITEEAVEFVKNMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRTRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAIDVKRFFEQVFTDQMKVVAFHDSTIDKYKSMLPMEHVVLIQDEEQAKACMFAGEQIVLLDLPPLQ
KWLECLFFQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=469732 H1W83_RS24425 WP_237892996.1 4658627..4660969(-) (recJ) [Priestia megaterium strain 2020WEIHUA_L]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCAGGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCGGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCTGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACGGATCACCACGAGCCGGGTCCGATTTTACCGGATGCGTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCGGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCTATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCACTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGTA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTAAAAATATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGTGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACGTTAAACAGCGA
TGATGTAGAAGAGCTGCGCACACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAATATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTGGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAACCGCAGCTGATGGTAGAA
GATATCTCTGTAGATGAGTGGCAGATGTTTGATTATCGCAGTGCTATTGATGTCAAACGTTTCTTTGAGCAGGTATTCAC
GGATCAAATGAAAGTTGTTGCTTTTCACGATTCAACAATTGATAAATACAAGTCGATGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATTGTTTTATTAGATTTGCCTCCTTTACAG
AAGTGGCTTGAATGTTTGTTTTTCCAAGGTATTCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCACTTTAAATGGTTCTACGGCTTTTTAGCGAAAAAGGGTCCTTTTGATTTAAAACGCT
ACAAAGAAGATTTAGCAATTCATAAGGGCTGGTCGAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHHA1 domain (DHHA1)

(305-439)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.128

100

0.551