Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   JQ456_RS24305 Genome accession   NZ_CP069337
Coordinates   5235530..5235955 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain E04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5230530..5240955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQ456_RS24290 (JQ456_24275) pilX 5231094..5231681 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  JQ456_RS24295 (JQ456_24280) pilY1 5231693..5235184 (+) 3492 WP_023089636.1 type 4a pilus biogenesis protein PilY1 -
  JQ456_RS24300 (JQ456_24285) pilY2 5235186..5235533 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  JQ456_RS24305 (JQ456_24290) comF 5235530..5235955 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  JQ456_RS24310 (JQ456_24295) ispH 5236002..5236946 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  JQ456_RS24315 (JQ456_24300) fkpB 5237032..5237472 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  JQ456_RS24320 (JQ456_24305) lspA 5237465..5237974 (-) 510 WP_003102615.1 signal peptidase II -
  JQ456_RS24325 (JQ456_24310) ileS 5237967..5240798 (-) 2832 WP_003119417.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=466259 JQ456_RS24305 WP_003094721.1 5235530..5235955(+) (comF) [Pseudomonas aeruginosa strain E04]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=466259 JQ456_RS24305 WP_003094721.1 5235530..5235955(+) (comF) [Pseudomonas aeruginosa strain E04]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383