Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   IS492_RS07720 Genome accession   NZ_CP069177
Coordinates   1667687..1668112 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain Z154     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1662687..1673112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS492_RS07700 (IS492_07695) ileS 1662844..1665675 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  IS492_RS07705 (IS492_07700) lspA 1665668..1666177 (+) 510 WP_003102615.1 signal peptidase II -
  IS492_RS07710 (IS492_07705) fkpB 1666170..1666610 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  IS492_RS07715 (IS492_07710) ispH 1666696..1667640 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  IS492_RS07720 (IS492_07715) comF 1667687..1668112 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  IS492_RS07725 (IS492_07720) pilY2 1668109..1668456 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  IS492_RS07730 (IS492_07725) pilY1 1668458..1671949 (-) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  IS492_RS07735 (IS492_07730) pilX 1671961..1672548 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=464285 IS492_RS07720 WP_003094721.1 1667687..1668112(-) (comF) [Pseudomonas aeruginosa strain Z154]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=464285 IS492_RS07720 WP_003094721.1 1667687..1668112(-) (comF) [Pseudomonas aeruginosa strain Z154]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383