Detailed information    

experimental Experimentally validated

Overview

Name   cbpD/crfP   Type   Auxiliary factor
Locus tag   ZY05719_RS10040 Genome accession   NZ_CP007497
Coordinates   2031471..2032385 (+) Length   304 a.a.
NCBI ID   WP_012775400.1    Uniprot ID   A0A0Z9LT74
Organism   Streptococcus suis strain ZY05719     
Function   cell lysis   
Cell lysis

Function

CrfP is a novel murein hydrolase that is widespread in S. suis and stimulated with a peptide pheromone in the competent state through a process controlled by ComX. Further characterization showed that CrfP could be exported to extracellular bacterial cells and lytic S. suis strains of different serotypes, and this finding was verified by TEM and a turbidity assay. Instead of stopping the natural transformation process, the inactivation of CrfP clearly reduced the effective transformation rate.


Genomic Context

Location: 2026471..2037385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ZY05719_RS10025 (ZY05719_10055) mutS 2026501..2029041 (-) 2541 WP_012775399.1 DNA mismatch repair protein MutS -
  ZY05719_RS10030 (ZY05719_10060) argR 2029056..2029496 (-) 441 WP_002937036.1 arginine repressor -
  ZY05719_RS10035 (ZY05719_10065) argS 2029603..2031291 (+) 1689 WP_012028003.1 arginine--tRNA ligase -
  ZY05719_RS10040 (ZY05719_10070) cbpD/crfP 2031471..2032385 (+) 915 WP_012775400.1 SH3 domain-containing protein Auxiliary factor
  ZY05719_RS10045 (ZY05719_10075) - 2032438..2032959 (-) 522 WP_012775401.1 hypothetical protein -
  ZY05719_RS10050 (ZY05719_10080) - 2033071..2033253 (+) 183 WP_002937045.1 hypothetical protein -
  ZY05719_RS10055 (ZY05719_10085) malQ 2033296..2034813 (-) 1518 WP_012028588.1 4-alpha-glucanotransferase -
  ZY05719_RS10060 (ZY05719_10090) - 2035115..2036374 (+) 1260 WP_002936107.1 extracellular solute-binding protein -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 33555.59 Da        Isoelectric Point: 9.3734

>NTDB_id=464 ZY05719_RS10040 WP_012775400.1 2031471..2032385(+) (cbpD/crfP) [Streptococcus suis strain ZY05719]
MKTFLRRKGIACQLLAAIFIVGINLQSPPSASANARGDDYPYTAVDAVDPWRLYTRQCTSFVAFRLSTVNGFTLPPAYGD
ANVWGHRAKQEGYQVNMTPAVGAVAWWETPMHVAWVSAVNGDTVEIEEYNYGVRYTYGRRVIPKNAVSGYIHFKNLGAGT
TNSQPALAPSGTYTFTSQLAIKSQASLSSPTLDYYYAGESVRYDKVLEDDGQRWISYISYSGARRYIAIQPTITQSTNNS
LPASGSYTIQSYSSVRNNPSLSSPEITFYGAGSVVRYDKVITAEGRTWISYISYSGARRYIAIS

Nucleotide


Download         Length: 915 bp        

>NTDB_id=464 ZY05719_RS10040 WP_012775400.1 2031471..2032385(+) (cbpD/crfP) [Streptococcus suis strain ZY05719]
ATGAAAACATTCTTGAGAAGAAAAGGAATCGCCTGTCAGCTTCTAGCCGCCATTTTTATTGTCGGTATCAATTTACAATC
CCCACCTTCAGCCTCAGCCAATGCAAGAGGAGATGACTATCCCTACACAGCAGTTGATGCAGTCGATCCATGGAGACTCT
ACACACGCCAGTGCACCTCCTTTGTAGCCTTTCGACTTAGCACAGTTAACGGTTTTACATTGCCACCTGCCTATGGTGAT
GCCAATGTTTGGGGCCATCGTGCCAAACAAGAAGGTTATCAAGTTAATATGACACCTGCTGTCGGAGCCGTTGCCTGGTG
GGAAACTCCCATGCATGTCGCATGGGTTTCTGCAGTAAATGGAGATACCGTAGAGATTGAGGAATACAACTACGGTGTTC
GCTATACCTACGGAAGGCGAGTTATCCCCAAAAATGCCGTTAGCGGCTACATTCATTTTAAAAACCTCGGAGCGGGAACA
ACCAACTCTCAACCCGCCTTAGCCCCAAGCGGCACCTATACCTTTACCAGCCAACTGGCCATCAAATCTCAAGCTAGTCT
ATCTAGCCCTACCCTCGATTACTATTATGCTGGCGAAAGCGTCCGCTACGACAAGGTTCTTGAAGACGACGGACAACGCT
GGATTAGCTATATCAGCTATTCTGGAGCTCGGCGCTATATCGCCATCCAGCCTACCATTACTCAATCTACAAACAACAGC
CTACCAGCATCAGGTAGCTACACCATCCAGTCCTACTCTAGTGTCCGCAACAATCCAAGTCTGTCCAGTCCTGAAATCAC
CTTCTACGGAGCTGGCTCAGTCGTTCGCTATGATAAAGTTATTACTGCCGAAGGTCGCACATGGATTAGCTATATCAGTT
ACTCAGGTGCTCGACGCTACATCGCCATCTCCTAA

Domains

Predicted by InterproScan.

Bacterial SH3 domain (SH3_5)

(168-229)

Bacterial SH3 domain (SH3_5)

(240-303)

CHAP domain (CHAP)

(54-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Z9LT74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cbpD Streptococcus pneumoniae D39

43.689

100

0.444
  cbpD Streptococcus pneumoniae TIGR4

43.689

100

0.444
  cbpD Streptococcus pneumoniae Rx1

43.689

100

0.444
  cbpD Streptococcus pneumoniae R6

43.689

100

0.444
  cbpD Streptococcus mitis SK321

48.133

79.276

0.382
  cbpD Streptococcus mitis NCTC 12261

48.927

76.645

0.375

Multiple sequence alignment    



References

[1] Edoardo Zaccaria et al. (2014) Control of competence for DNA transformation in streptococcus suis by genetically transferable pherotypes. PloS One 9(6):e99394. [PMID: 24968201]