Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   HW576_RS23590 Genome accession   NZ_CP058262
Coordinates   4545026..4547368 (-) Length   780 a.a.
NCBI ID   WP_098399860.1    Uniprot ID   -
Organism   Priestia megaterium strain BIM B-1314D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4540026..4552368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW576_RS23565 (HW576_23565) - 4540419..4540595 (-) 177 WP_013059305.1 hypothetical protein -
  HW576_RS23570 (HW576_23570) - 4540762..4541646 (+) 885 WP_223268964.1 N-acetylmuramoyl-L-alanine amidase -
  HW576_RS23575 (HW576_23575) dtd 4541637..4542098 (-) 462 WP_177563878.1 D-aminoacyl-tRNA deacylase -
  HW576_RS23580 (HW576_23580) - 4542114..4544312 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  HW576_RS23585 (HW576_23585) - 4544498..4545010 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  HW576_RS23590 (HW576_23590) recJ 4545026..4547368 (-) 2343 WP_098399860.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  HW576_RS23595 (HW576_23595) secDF 4547509..4549746 (-) 2238 WP_013059311.1 protein translocase subunit SecDF -
  HW576_RS23600 (HW576_23600) - 4549884..4550177 (-) 294 WP_013059312.1 post-transcriptional regulator -
  HW576_RS23605 (HW576_23605) spoVB 4550301..4551851 (+) 1551 WP_025601348.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87875.49 Da        Isoelectric Point: 4.7742

>NTDB_id=463324 HW576_RS23590 WP_098399860.1 4545026..4547368(-) (recJ) [Priestia megaterium strain BIM B-1314D]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDTLNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQIFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEQAKACMFAGEQIVLLDLPPSR
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=463324 HW576_RS23590 WP_098399860.1 4545026..4547368(-) (recJ) [Priestia megaterium strain BIM B-1314D]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCATGATATGGACAAAGCAGTGGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATTTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAATTA
AACTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTCGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACTAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATTGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCCGATC
CAGCTGTTCATTTGCTTATGACGACAAATGCTGAAGAGGCAAAAGGCTTAGCTAAAGAAATTGATACGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAGGAAGCCGTTGAATTTGTTGAGAGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAATTCTATAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGTTTTGACTTATTC
GCGAACTTATCTAAGTGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATATTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACGG
AAATGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.769

100

0.558