Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HWV68_RS13350 Genome accession   NZ_CP058242
Coordinates   2557988..2559025 (-) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus subtilis strain SP1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2552988..2564025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV68_RS13300 (HWV68_13295) tasA 2553086..2553871 (-) 786 WP_004398632.1 biofilm matrix protein TasA -
  HWV68_RS13305 (HWV68_13300) sipW 2553935..2554507 (-) 573 WP_003246088.1 signal peptidase I SipW -
  HWV68_RS13310 (HWV68_13305) tapA 2554491..2555252 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  HWV68_RS13315 (HWV68_13310) yqzG 2555524..2555850 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  HWV68_RS13320 (HWV68_13315) spoIITA 2555892..2556071 (-) 180 WP_003230176.1 YqzE family protein -
  HWV68_RS13325 (HWV68_13320) comGG 2556142..2556516 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  HWV68_RS13330 (HWV68_13325) comGF 2556517..2556900 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  HWV68_RS13335 (HWV68_13330) comGE 2556926..2557273 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  HWV68_RS13340 (HWV68_13335) comGD 2557257..2557688 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  HWV68_RS13345 (HWV68_13340) comGC 2557678..2557974 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  HWV68_RS13350 (HWV68_13345) comGB 2557988..2559025 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  HWV68_RS13355 (HWV68_13350) comGA 2559012..2560082 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  HWV68_RS13360 (HWV68_13355) corA 2560494..2561447 (-) 954 WP_004399136.1 magnesium transporter CorA -
  HWV68_RS13365 (HWV68_13360) yqhB 2561590..2562918 (+) 1329 WP_009967730.1 hemolysin family protein -
  HWV68_RS13370 (HWV68_13365) rsbRD 2562971..2563807 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=462947 HWV68_RS13350 WP_009967727.1 2557988..2559025(-) (comGB) [Bacillus subtilis strain SP1]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=462947 HWV68_RS13350 WP_009967727.1 2557988..2559025(-) (comGB) [Bacillus subtilis strain SP1]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936