Detailed information NTG0046118

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: LILO_RS09805
• Genome accession: NC_020450
• Coordinates: 2053997..2056336 (-)
• Length: 779 a.a.
• NCBI ID: WP_015427047.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis IO-1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2048997..2061336

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LILO_RS09765 (lilo_1893)	-	2049913..2050419 (-)	507	WP_012898444.1	Asp23/Gls24 family envelope stress response protein	-
LILO_RS09770	-	2050440..2050628 (-)	189	WP_012898445.1	DUF2273 domain-containing protein	-
LILO_RS09775 (lilo_1894)	amaP	2050639..2051199 (-)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
LILO_RS09780 (lilo_1895)	-	2051227..2051469 (-)	243	WP_015427043.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
LILO_RS09785 (lilo_1896)	fmt	2051644..2052603 (-)	960	WP_015427044.1	methionyl-tRNA formyltransferase	-
LILO_RS09790 (lilo_1897)	-	2052600..2053064 (-)	465	WP_003131474.1	VOC family protein	-
LILO_RS09795 (lilo_1898)	-	2053030..2053551 (-)	522	WP_015427045.1	NUDIX hydrolase	-
LILO_RS09800 (lilo_1899)	-	2053564..2053938 (-)	375	WP_042230606.1	nuclear transport factor 2 family protein	-
LILO_RS09805 (lilo_1900)	priA	2053997..2056336 (-)	2340	WP_015427047.1	primosomal protein N'	Machinery gene
LILO_RS09810 (lilo_1901)	rpoZ	2056475..2056828 (-)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
LILO_RS09815 (lilo_1902)	gmk	2057008..2057625 (-)	618	WP_003131468.1	guanylate kinase	-
LILO_RS09820 (lilo_1903)	rny	2057772..2059367 (-)	1596	WP_015427048.1	ribonuclease Y	-
LILO_RS09825 (lilo_1904)	-	2059598..2060311 (-)	714	WP_015427049.1	glucosamine-6-phosphate deaminase	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88914.25 Da
• Isoelectric Point: 6.6749

>NTDB_id=46118 LILO_RS09805 WP_015427047.1 2053997..2056336(-) (priA) [Lactococcus lactis subsp. lactis IO-1]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENR
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVKSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEGVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSHSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVFIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=46118 LILO_RS09805 WP_015427047.1 2053997..2056336(-) (priA) [Lactococcus lactis subsp. lactis IO-1]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTCCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTACGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAGA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTACGACTTCAAGAAGAAAAAATTGCTGTCAAATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAGGTTGAAATTTCTAGGACGAAAGAAAAATTTGAAGGTGTGGAA
AATGACAGTGCCAAAATTCTCAACTCCGAACAAAATGCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCCGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACGAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTCTCAC
ACTCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGCCGCGAATGTGGTTTTGTTGTTGATTGTCCTAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATTATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACAACTCGGACAAAAAATGCTCATGAAAAAATTCTTGAAAAATTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTAATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTCTTCATTCAAACTTTCAATCCACATCATTACGCCATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAGGCTAAAATTTTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATTTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGCGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

• Type III restriction enzyme, res subunit (ResIII) (247-405)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.512	100	0.49