Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   HUW50_RS01530 Genome accession   NZ_CP055263
Coordinates   299636..301996 (-) Length   786 a.a.
NCBI ID   WP_066334455.1    Uniprot ID   -
Organism   Metabacillus sp. KUDC1714     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 294636..306996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW50_RS01515 (HUW50_01515) dtd 295947..296387 (-) 441 WP_066334467.1 D-aminoacyl-tRNA deacylase -
  HUW50_RS01520 (HUW50_01520) - 296402..298609 (-) 2208 WP_066334461.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  HUW50_RS01525 (HUW50_01525) - 299107..299619 (-) 513 WP_066334459.1 adenine phosphoribosyltransferase -
  HUW50_RS01530 (HUW50_01530) recJ 299636..301996 (-) 2361 WP_066334455.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  HUW50_RS01535 (HUW50_01535) - 302091..302408 (-) 318 WP_066334454.1 lipopolysaccharide assembly LapA domain-containing protein -
  HUW50_RS01540 (HUW50_01540) secDF 302692..304935 (-) 2244 WP_066334450.1 protein translocase subunit SecDF -
  HUW50_RS01545 (HUW50_01545) - 305064..305357 (-) 294 WP_066334443.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88253.09 Da        Isoelectric Point: 5.1349

>NTDB_id=457281 HUW50_RS01530 WP_066334455.1 299636..301996(-) (recJ) [Metabacillus sp. KUDC1714]
MLKAKTRWMVQPSEDTLMKTFIDNLSITPLVASLLVNRGINTIEKAREFLQTKQQTFHDPFLLKDMDKAVHRIKTAIENR
EHILVYGDYDADGVSSTTVLLTTLSKLGAQADFYIPNRFTEGYGPNETAFRQVHEKGFSLIITVDTGIAAVHEAEVAKEL
GIDLIITDHHEPGPVLPDALAIIHPKQPGCPYPFKELAGVGVAFKLSHALLGELPADLLEVAAIGTIADLVTLHGENRLI
AKIGIEQLKSTSRTGLKALLKVAKVTNSDINEDTIGFALAPRINAVGRLQSADPAVDLLLTEDAEEAIEIAQEIDLLNKE
RQKLVSTMTEEAIEQVESSFPITDNPVLVIAKEGWNPGVVGIVASRLVERYYRPTIVLSVDKEKGVAKGSARSIIGFDLF
ENLSTCRDILPHFGGHPMAAGMTLELEDVDLLRQRLVEKAKTILTDEDFTPITKVDVSCKLEDISVNSIEEMQLLAPFGM
HNPKPIIQVEDVTLANIRKIGAEQNHLKLVFEQEEHQLDSVGFGFGYIHDDLSPTVTLSAIGELSINEWNNFRKPQLMLQ
DVKVDQWQLFDYRGTRNVDKLLKNVNLVKNSTIITFQSAIYEDLKQKGFDTNTIFVNTVSVAQNLNSKNKNLVLMDIPSS
LDLLDSLFVQGKPDRIYTVFLQEADHFFATIPTRDHFKWYYSFLLKKGSFKLKEQGEQLAKHKGWSKETIEFMSQVFFEL
EFVTIENGVISTNSTSRKRDLAESKTYAQKQMQIELEKTLLYTSYMQLKQWFEERFQSTADTFVNV

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=457281 HUW50_RS01530 WP_066334455.1 299636..301996(-) (recJ) [Metabacillus sp. KUDC1714]
ATGTTAAAGGCAAAAACAAGATGGATGGTTCAGCCATCTGAAGATACATTAATGAAAACATTTATAGATAACTTATCGAT
TACACCACTAGTTGCATCATTACTTGTTAATCGTGGGATAAATACAATTGAAAAAGCACGTGAATTTTTACAAACAAAGC
AACAGACTTTTCATGATCCTTTCCTTTTAAAAGATATGGACAAAGCTGTCCACCGAATAAAAACAGCAATCGAAAACAGA
GAACATATATTAGTATACGGTGATTATGATGCGGATGGCGTAAGTAGTACAACTGTTTTGTTAACTACTTTAAGCAAGTT
GGGAGCACAAGCTGACTTTTATATCCCGAATCGTTTTACAGAAGGATATGGCCCTAATGAAACGGCTTTTAGACAGGTAC
ATGAGAAGGGATTTTCACTGATTATCACTGTTGACACGGGAATTGCAGCTGTTCATGAGGCTGAAGTTGCAAAAGAGCTT
GGTATTGACCTCATCATCACTGACCACCATGAACCAGGGCCGGTTTTGCCTGATGCGTTAGCGATTATCCATCCAAAGCA
ACCTGGATGTCCTTATCCATTTAAGGAATTGGCAGGAGTTGGAGTTGCATTTAAATTAAGCCACGCACTTTTGGGTGAAC
TCCCAGCAGATTTATTAGAGGTCGCAGCAATTGGCACCATCGCTGATCTAGTGACTCTACATGGGGAAAATAGATTAATT
GCAAAAATCGGAATTGAACAATTAAAATCAACAAGTCGTACGGGGCTAAAAGCATTGTTAAAGGTAGCCAAGGTTACAAA
CTCTGACATTAACGAAGATACAATTGGTTTTGCGTTAGCACCTAGAATTAATGCAGTTGGCCGGTTACAATCTGCAGATC
CAGCTGTAGACTTACTTTTAACAGAAGATGCAGAAGAAGCAATTGAAATTGCGCAGGAAATAGATTTATTAAATAAAGAG
CGACAAAAGCTAGTAAGCACTATGACAGAGGAAGCGATTGAACAAGTAGAATCAAGTTTTCCGATCACTGATAATCCTGT
GCTTGTCATAGCGAAGGAAGGCTGGAATCCTGGGGTTGTAGGAATTGTTGCATCACGACTTGTTGAACGTTATTATCGAC
CAACGATTGTTCTAAGCGTTGATAAAGAAAAAGGCGTCGCGAAAGGTTCTGCAAGAAGTATTATTGGGTTCGATTTATTC
GAAAATCTTTCAACCTGTCGAGATATTTTACCGCATTTTGGTGGTCATCCAATGGCCGCAGGTATGACACTAGAACTTGA
AGATGTCGATTTATTAAGGCAAAGATTAGTTGAAAAAGCAAAAACTATTTTAACAGATGAAGACTTTACACCAATTACAA
AGGTTGATGTCTCCTGTAAATTAGAAGATATCTCAGTAAATTCAATTGAAGAGATGCAGCTTCTTGCACCGTTTGGTATG
CACAATCCAAAGCCAATTATTCAGGTTGAGGATGTCACGTTAGCAAACATACGAAAAATTGGTGCAGAACAAAATCATCT
AAAGCTTGTTTTTGAACAAGAAGAACATCAACTTGATAGTGTTGGATTTGGATTTGGTTATATTCATGATGATCTTTCAC
CTACTGTTACATTATCTGCAATTGGGGAATTATCTATTAATGAGTGGAATAATTTTCGAAAACCGCAATTAATGCTACAG
GATGTAAAAGTAGATCAATGGCAACTGTTTGACTATCGTGGCACACGTAATGTTGACAAGCTTTTAAAAAATGTTAATTT
AGTAAAAAATAGTACTATTATTACATTTCAGTCAGCTATATATGAGGATTTGAAACAAAAAGGTTTTGATACGAATACAA
TCTTTGTTAATACTGTATCTGTCGCTCAAAACCTAAATAGCAAAAATAAAAACCTTGTATTAATGGATATACCGTCATCA
CTAGACTTGCTGGATTCCCTTTTTGTTCAAGGAAAGCCAGATCGAATCTATACTGTTTTTTTACAAGAAGCCGATCACTT
CTTTGCAACAATACCAACTAGAGATCACTTTAAATGGTATTACAGCTTTTTATTAAAGAAAGGTTCCTTTAAATTAAAGG
AACAAGGTGAACAACTTGCCAAACACAAAGGTTGGTCAAAAGAAACAATAGAGTTCATGTCACAGGTGTTTTTTGAGCTA
GAATTTGTTACAATAGAAAATGGTGTTATTTCGACAAATTCTACGTCTCGAAAAAGAGATTTAGCTGAATCGAAAACATA
TGCACAAAAGCAAATGCAAATTGAATTAGAAAAAACGTTATTATATACATCTTACATGCAGTTAAAACAGTGGTTTGAAG
AGAGATTTCAATCAACAGCTGATACGTTTGTAAATGTATGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-772)

DHHA1 domain (DHHA1)

(311-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

57.727

99.618

0.575