Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JHL13_RS02060 Genome accession   NZ_CP066844
Coordinates   457730..458197 (-) Length   155 a.a.
NCBI ID   WP_022646190.1    Uniprot ID   -
Organism   Escherichia coli strain Z30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452730..463197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHL13_RS02035 (JHL13_02000) rpsG 452871..453341 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  JHL13_RS02040 (JHL13_02005) fusA 453438..455552 (+) 2115 WP_000124700.1 elongation factor G -
  JHL13_RS02045 (JHL13_02010) tuf 455623..456807 (+) 1185 WP_000031783.1 elongation factor Tu -
  JHL13_RS02050 (JHL13_02015) bfd 456990..457184 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  JHL13_RS02055 (JHL13_02020) bfr 457257..457733 (+) 477 WP_000675515.1 bacterioferritin -
  JHL13_RS02060 (JHL13_02025) pilD 457730..458197 (-) 468 WP_022646190.1 prepilin peptidase Machinery gene
  JHL13_RS02065 (JHL13_02030) rpsJ 458576..458887 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  JHL13_RS02070 (JHL13_02035) rplC 458920..459549 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  JHL13_RS02075 (JHL13_02040) rplD 459560..460165 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  JHL13_RS02080 (JHL13_02045) rplW 460162..460464 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  JHL13_RS02085 (JHL13_02050) rplB 460482..461303 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  JHL13_RS02090 (JHL13_02055) rpsS 461320..461598 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  JHL13_RS02095 (JHL13_02060) rplV 461613..461945 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  JHL13_RS02100 (JHL13_02065) rpsC 461963..462664 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  JHL13_RS02105 (JHL13_02070) rplP 462677..463087 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17064.20 Da        Isoelectric Point: 8.1255

>NTDB_id=456665 JHL13_RS02060 WP_022646190.1 457730..458197(-) (pilD) [Escherichia coli strain Z30]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLATAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=456665 JHL13_RS02060 WP_022646190.1 457730..458197(-) (pilD) [Escherichia coli strain Z30]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATTCTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGACTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368