Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   HUK70_RS26430 Genome accession   NZ_CP054789
Coordinates   5542133..5543470 (+) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa strain SE5381     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5537133..5548470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK70_RS26405 (HUK70_26235) pgeF 5537276..5538004 (-) 729 WP_003102588.1 peptidoglycan editing factor PgeF -
  HUK70_RS26410 (HUK70_26240) rluD 5538001..5538963 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  HUK70_RS26415 (HUK70_26245) - 5539109..5540134 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  HUK70_RS26420 (HUK70_26250) - 5540291..5540536 (+) 246 WP_003102591.1 PP0621 family protein -
  HUK70_RS26425 (HUK70_26255) pilS 5540526..5542118 (+) 1593 WP_003123394.1 two-component system sensor histidine kinase PilS Regulator
  HUK70_RS26430 (HUK70_26260) pilR 5542133..5543470 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  HUK70_RS26435 (HUK70_26265) thiO 5543516..5544610 (-) 1095 WP_003119424.1 glycine oxidase ThiO -
  HUK70_RS26440 (HUK70_26270) - 5544745..5545254 (+) 510 WP_003102595.1 Tfp pilus assembly protein FimT/FimU -
  HUK70_RS26445 (HUK70_26275) - 5545360..5545866 (+) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  HUK70_RS26450 (HUK70_26280) pilV 5545857..5546414 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  HUK70_RS26455 (HUK70_26285) pilW 5546411..5547235 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  HUK70_RS26460 (HUK70_26290) pilX 5547232..5547819 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=454573 HUK70_RS26430 WP_003094694.1 5542133..5543470(+) (pilR) [Pseudomonas aeruginosa strain SE5381]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=454573 HUK70_RS26430 WP_003094694.1 5542133..5543470(+) (pilR) [Pseudomonas aeruginosa strain SE5381]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

100

100

1
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384