Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HUF97_RS12055 Genome accession   NZ_CP054562
Coordinates   2518769..2519383 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain YB-130     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513769..2524383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUF97_RS12025 rpsT 2514072..2514338 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  HUF97_RS12030 holA 2514355..2515396 (-) 1042 Protein_2344 DNA polymerase III subunit delta -
  HUF97_RS12035 - 2515436..2515587 (-) 152 Protein_2345 hypothetical protein -
  HUF97_RS12040 - 2515628..2515762 (+) 135 WP_003152870.1 YqzM family protein -
  HUF97_RS12045 comEC 2515781..2518132 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HUF97_RS12050 - 2518133..2518702 (-) 570 WP_003152868.1 ComE operon protein 2 -
  HUF97_RS12055 comEA 2518769..2519383 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  HUF97_RS12060 comER 2519442..2520263 (+) 822 WP_012118027.1 late competence protein ComER -
  HUF97_RS12065 - 2520332..2521069 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  HUF97_RS12070 rsfS 2521066..2521422 (-) 357 WP_007408260.1 ribosome silencing factor -
  HUF97_RS12075 yqeK 2521426..2522001 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HUF97_RS12080 - 2521991..2522560 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  HUF97_RS12085 yhbY 2522571..2522861 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  HUF97_RS12090 aroE 2522855..2523697 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=453378 HUF97_RS12055 WP_032873955.1 2518769..2519383(-) (comEA) [Bacillus velezensis strain YB-130]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=453378 HUF97_RS12055 WP_032873955.1 2518769..2519383(-) (comEA) [Bacillus velezensis strain YB-130]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363