Detailed information NTG0451164

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: HRE60_RS04300
• Genome accession: NZ_CP054153
• Coordinates: 883810..884691 (+)
• Length: 293 a.a.
• NCBI ID: WP_181671213.1
• Uniprot ID: A0A7L6WJE6
• Organism: Streptococcus salivarius strain DB-B5
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 878810..889691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HRE60_RS04275 (HRE60_04275)	-	879343..880764 (+)	1422	WP_181671210.1	DUF4832 domain-containing protein	-
HRE60_RS04280 (HRE60_04280)	galE	880779..881792 (+)	1014	WP_021144269.1	UDP-glucose 4-epimerase GalE	-
HRE60_RS04285 (HRE60_04285)	-	881984..882220 (+)	237	WP_181671211.1	DUF1146 family protein	-
HRE60_RS04290 (HRE60_04290)	murA	882288..883559 (+)	1272	WP_037611666.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
HRE60_RS04295 (HRE60_04295)	-	883561..883749 (+)	189	WP_181671212.1	DNA-directed RNA polymerase subunit beta	-
HRE60_RS04300 (HRE60_04300)	endA	883810..884691 (+)	882	WP_181671213.1	DNA/RNA non-specific endonuclease	Machinery gene
HRE60_RS04305 (HRE60_04305)	-	884768..885418 (-)	651	WP_002886253.1	amino acid ABC transporter permease	-
HRE60_RS04310 (HRE60_04310)	-	885430..886128 (-)	699	WP_002886251.1	amino acid ABC transporter permease	-
HRE60_RS04315 (HRE60_04315)	-	886146..886979 (-)	834	WP_181671214.1	transporter substrate-binding domain-containing protein	-
HRE60_RS04320 (HRE60_04320)	-	886991..887758 (-)	768	WP_104020925.1	amino acid ABC transporter ATP-binding protein	-
HRE60_RS04325 (HRE60_04325)	-	887947..888597 (-)	651	WP_181671215.1	amino acid ABC transporter permease	-
HRE60_RS04330 (HRE60_04330)	-	888609..889307 (-)	699	WP_002886251.1	amino acid ABC transporter permease	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31504.12 Da
• Isoelectric Point: 10.2177

>NTDB_id=451164 HRE60_RS04300 WP_181671213.1 883810..884691(+) (endA) [Streptococcus salivarius strain DB-B5]
MAKRKTSLTNKQKRQLVSLLIAVLIAVLGYIGTSNKLSPNNPIKQVASLVSGKSNNSVKSNGSTSGQSTPQEQLAETVMT
SSVKSQLGNAIEWNGAGAYIVNGNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVHNLSGEYSHAVDRGHLLGYALVGGLKGFDASTSNPENIAVQTAWANQANSETSTGQNYYESLVRKAQDKNKRVR
YRVTLIYEGDNLIPSGSHLEAKSADGSLEFNVFVPNVQQGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=451164 HRE60_RS04300 WP_181671213.1 883810..884691(+) (endA) [Streptococcus salivarius strain DB-B5]
ATGGCAAAAAGAAAGACATCTTTAACAAATAAACAGAAACGTCAGCTAGTCTCGTTACTAATAGCGGTCTTGATTGCAGT
TTTAGGTTACATTGGAACGAGCAATAAGCTCTCACCTAACAATCCAATCAAGCAGGTAGCTAGTCTAGTCTCGGGAAAAT
CCAATAACTCCGTCAAATCAAACGGCTCAACAAGTGGTCAGTCTACGCCACAAGAGCAGCTCGCTGAGACGGTGATGACC
AGCTCCGTCAAGAGTCAACTAGGAAACGCCATAGAATGGAATGGTGCTGGTGCCTATATTGTTAACGGTAATAAAACCAA
TCTTAATGCTAAGGTATCTAGCCAACCCTATGCTAATAACCAAGTTAAAACGGTTCAAGGACAAACGGTTCCTACAGTTG
CGAATGCCTTGTTAAGTAAAGCGACCAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGCTCAACATCTTGGACACCA
GCTGGATGGCATCAAGTTCACAATCTTTCAGGAGAGTATAGCCATGCCGTAGACCGAGGCCATTTGTTGGGCTATGCACT
TGTAGGTGGTCTCAAAGGATTTGATGCCTCGACTTCAAATCCAGAAAATATTGCCGTTCAAACTGCTTGGGCTAACCAAG
CTAATAGCGAAACTTCCACAGGCCAAAACTATTATGAAAGCCTGGTACGAAAAGCTCAGGATAAAAACAAACGTGTCCGT
TATCGCGTTACCCTCATTTATGAAGGAGATAACCTGATTCCATCAGGATCACATCTTGAAGCCAAGTCAGCAGATGGCTC
GCTTGAGTTCAACGTCTTCGTTCCAAATGTCCAACAGGGAATTACCCTAGATTATTATTCAGGGAAAGTAACGGTGAATT
AA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (99-266)

3D structure

• AlphaFold DB: A0A7L6WJE6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	70.356	86.348	0.608
endA	Streptococcus pneumoniae D39	70.356	86.348	0.608
endA	Streptococcus pneumoniae R6	70.356	86.348	0.608
endA	Streptococcus pneumoniae TIGR4	70.356	86.348	0.608