Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   I6G22_RS03340 Genome accession   NZ_CP065737
Coordinates   617698..618477 (-) Length   259 a.a.
NCBI ID   WP_050428201.1    Uniprot ID   -
Organism   Lactococcus lactis strain FDAARGOS_865     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 612698..623477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G22_RS03320 (I6G22_03320) pflA 613423..614217 (-) 795 WP_003130318.1 pyruvate formate-lyase-activating protein -
  I6G22_RS03325 (I6G22_03325) - 614435..615772 (-) 1338 WP_021722264.1 hemolysin family protein -
  I6G22_RS03330 (I6G22_03330) - 615894..617009 (-) 1116 WP_023189057.1 AI-2E family transporter -
  I6G22_RS03335 (I6G22_03335) - 617158..617607 (+) 450 WP_025017176.1 GNAT family N-acetyltransferase -
  I6G22_RS03340 (I6G22_03340) endA 617698..618477 (-) 780 WP_050428201.1 DNA/RNA non-specific endonuclease Machinery gene
  I6G22_RS03345 (I6G22_03345) - 618671..619567 (-) 897 WP_032943636.1 peptidoglycan amidohydrolase family protein -
  I6G22_RS03350 (I6G22_03350) - 619679..619933 (-) 255 WP_023189062.1 phage holin -
  I6G22_RS03355 (I6G22_03355) - 619923..620225 (-) 303 WP_025017161.1 hypothetical protein -
  I6G22_RS03360 (I6G22_03360) - 620430..620723 (+) 294 WP_025017162.1 hypothetical protein -
  I6G22_RS03365 (I6G22_03365) - 620753..621004 (-) 252 WP_032943638.1 hypothetical protein -
  I6G22_RS03370 (I6G22_03370) - 621076..622992 (-) 1917 WP_052484082.1 glycoside hydrolase domain-containing protein -
  I6G22_RS03375 (I6G22_03375) - 623092..623439 (-) 348 WP_023189066.1 DUF722 domain-containing protein -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29129.05 Da        Isoelectric Point: 9.0583

>NTDB_id=450995 I6G22_RS03340 WP_050428201.1 617698..618477(-) (endA) [Lactococcus lactis strain FDAARGOS_865]
MKNKKIIPLLIVSIFILGGCANSQSDMDKSNHKWSQKDKTPSEELAKSVLTSSIKEQLKTDNIKFDHGSFIVNNNNNDLA
IINVVDPYAKNEIDIQGRPYLGEAVLPKSIQNNEKDNKDEKVIPQFGWGQKSNLKGFYKKAYTKGFLLNSKLIGGVKDFN
DSGSNMENIFTQTVWASEANDSSSKGQKYFENMIHKVQDKNMIIKYRVKALYDGSNLVPSGTEIEAKSDDFSLEIHVFIP
NVQPNLKVDYKTGQVTEVK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=450995 I6G22_RS03340 WP_050428201.1 617698..618477(-) (endA) [Lactococcus lactis strain FDAARGOS_865]
ATGAAGAACAAAAAAATTATCCCATTACTGATTGTCAGCATTTTTATCTTAGGGGGCTGTGCTAACAGTCAATCTGATAT
GGATAAAAGTAATCATAAATGGAGTCAAAAAGATAAGACACCCTCAGAAGAACTCGCAAAAAGTGTTCTGACTTCTTCAA
TAAAAGAACAATTAAAAACAGATAACATAAAATTTGATCACGGTTCATTTATCGTTAATAATAATAACAATGATTTAGCA
ATTATTAATGTTGTAGACCCATACGCTAAGAATGAAATAGATATTCAAGGGCGTCCTTATTTGGGTGAAGCAGTTCTCCC
AAAATCTATTCAAAATAATGAAAAGGATAATAAAGATGAAAAAGTGATTCCTCAATTTGGTTGGGGACAAAAATCTAACT
TGAAAGGCTTCTATAAAAAAGCTTATACTAAAGGTTTTTTGCTAAATAGCAAACTAATAGGTGGCGTTAAAGATTTCAAT
GATAGCGGGTCAAATATGGAAAATATATTTACTCAAACGGTTTGGGCGAGTGAAGCTAATGATTCAAGTTCCAAGGGACA
AAAATATTTTGAAAATATGATTCATAAAGTTCAAGATAAGAATATGATCATCAAGTATAGAGTGAAAGCACTCTATGATG
GAAGTAACCTTGTCCCATCAGGAACTGAAATAGAAGCAAAATCTGATGATTTTTCACTCGAGATACATGTTTTTATTCCA
AATGTTCAACCAAATCTTAAAGTAGATTATAAAACAGGTCAGGTCACTGAAGTCAAATAA

Domains

Predicted by InterProScan.

DNA/RNA non-specific endonuclease (Endonuclea_NS_2)

(92-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

42.174

88.803

0.375
  endA Streptococcus pneumoniae D39

42.174

88.803

0.375
  endA Streptococcus pneumoniae R6

42.174

88.803

0.375
  endA Streptococcus pneumoniae TIGR4

42.174

88.803

0.375