Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FOC66_RS04965 Genome accession   NZ_CP053939
Coordinates   1051120..1052250 (+) Length   376 a.a.
NCBI ID   WP_003747246.1    Uniprot ID   -
Organism   Neisseria mucosa strain FDAARGOS_758     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1046120..1057250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOC66_RS04945 (FOC66_04945) - 1046337..1047215 (+) 879 WP_141745643.1 DMT family transporter -
  FOC66_RS04950 (FOC66_04950) lepB 1047270..1048289 (-) 1020 WP_003747241.1 signal peptidase I -
  FOC66_RS04955 (FOC66_04955) lepA 1048289..1050082 (-) 1794 WP_002226499.1 translation elongation factor 4 -
  FOC66_RS04960 (FOC66_04960) - 1050215..1050916 (-) 702 WP_003747243.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  FOC66_RS04965 (FOC66_04965) pilU 1051120..1052250 (+) 1131 WP_003747246.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FOC66_RS04970 (FOC66_04970) - 1052331..1053737 (-) 1407 WP_003747249.1 dihydrolipoyl dehydrogenase -
  FOC66_RS04975 (FOC66_04975) - 1053990..1054727 (-) 738 WP_003747251.1 redoxin family protein -
  FOC66_RS04980 (FOC66_04980) - 1054918..1055703 (-) 786 WP_003747252.1 1-acylglycerol-3-phosphate O-acyltransferase -
  FOC66_RS04985 (FOC66_04985) mutM 1055767..1056594 (-) 828 WP_003747256.1 DNA-formamidopyrimidine glycosylase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42437.58 Da        Isoelectric Point: 5.9015

>NTDB_id=449255 FOC66_RS04965 WP_003747246.1 1051120..1052250(+) (pilU) [Neisseria mucosa strain FDAARGOS_758]
MFDENTPGAKEELFTWLRHMNKYKGSDLFITTNFPPAMKVDGKIMRLTDEPLSAEKCMEIAFSIMSSKQIEEFSSTNECN
FAISLPDTSRFRVNAMVQRGATALVFRSITSNIPKFETLNLPPILKDIALRKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFDHIITIEDPIEFIHQHKNCIITQREIGVDTENWPIALKNTLRQAPDVILIGEIRDRETMDYAISFAETGHLCMATLHA
NSTNQALDRIINFFPEERRNQLLTDLSLNLQAFISQRLIPREGGRGRVAAVEVLLNSPIIADLIRKGEVHNIKEMMKKST
GMGMITFDQALYDLYENGDISYQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=449255 FOC66_RS04965 WP_003747246.1 1051120..1052250(+) (pilU) [Neisseria mucosa strain FDAARGOS_758]
ATGTTTGACGAAAATACTCCGGGCGCAAAAGAAGAATTGTTCACTTGGCTACGCCACATGAACAAATACAAAGGCTCCGA
CCTTTTCATTACCACCAATTTCCCGCCGGCCATGAAAGTGGACGGCAAAATCATGCGGCTGACCGATGAGCCGTTAAGCG
CTGAAAAATGTATGGAAATCGCTTTTTCGATTATGTCTTCCAAACAAATCGAAGAATTTTCCTCTACCAACGAATGCAAC
TTTGCCATCAGCCTGCCCGATACCAGCCGCTTCCGTGTCAATGCCATGGTTCAACGCGGCGCAACTGCTTTGGTATTCCG
CTCCATTACCAGCAATATCCCTAAATTTGAAACGCTCAATCTGCCGCCGATTCTGAAAGACATCGCCCTCAGAAAACGCG
GTCTGGTTATTTTTGTCGGCGGTACAGGTTCGGGCAAGTCCACTTCCCTCGCCTCCCTTATCGACTATCGAAACGAAAAC
AGCTTTGACCACATTATCACCATCGAAGATCCGATTGAATTTATCCACCAACATAAAAACTGCATTATCACCCAACGCGA
AATCGGCGTGGATACCGAAAATTGGCCGATTGCGCTGAAAAATACCCTGCGCCAAGCACCGGATGTCATTCTGATTGGCG
AAATCCGCGACCGCGAGACCATGGACTACGCCATTTCCTTCGCGGAAACCGGCCATTTGTGTATGGCGACACTGCATGCC
AACAGCACCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGTAACCAATTGCTAACCGATTTATC
GCTCAACCTGCAAGCCTTTATTTCACAACGCCTGATTCCCCGCGAAGGCGGACGGGGACGCGTAGCGGCTGTCGAGGTTT
TGCTCAATTCGCCCATTATTGCCGACTTGATTCGAAAAGGCGAAGTCCACAACATCAAAGAAATGATGAAAAAATCAACC
GGCATGGGCATGATTACTTTTGACCAGGCCTTGTATGATTTGTATGAAAACGGCGATATCAGCTATCAAGACGCTATTAA
AAATGCCGACTCCGCACACGATTTGCGCCTAGACATTCAATTACGAAGTCGCCGTGCACAAAATGCCGGCCCGGATTTGG
AATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.08

93.617

0.572
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

53.482

95.479

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.844

88.564

0.388
  pilT Acinetobacter baumannii strain A118

43.519

86.17

0.375
  pilT Acinetobacter baumannii D1279779

43.519

86.17

0.375
  pilT Acinetobacter nosocomialis M2

43.519

86.17

0.375
  pilT Acinetobacter baylyi ADP1

42.378

87.234

0.37
  pilT Legionella pneumophila strain Lp02

42.593

86.17

0.367
  pilT Legionella pneumophila strain ERS1305867

42.593

86.17

0.367