Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY15_RS01300 Genome accession   NZ_CP053800
Coordinates   278732..279748 (+) Length   338 a.a.
NCBI ID   WP_175235514.1    Uniprot ID   -
Organism   Vibrio cholerae strain SO5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273732..284748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY15_RS01285 (HPY15_01285) - 274273..275004 (-) 732 WP_000495223.1 glutathione peroxidase -
  HPY15_RS01290 (HPY15_01290) oxyR 275144..276037 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  HPY15_RS01295 (HPY15_01295) - 276112..278589 (-) 2478 WP_046126012.1 penicillin-binding protein 1A -
  HPY15_RS01300 (HPY15_01300) pilM 278732..279748 (+) 1017 WP_175235514.1 type IV pilus assembly protein PilM Machinery gene
  HPY15_RS01305 (HPY15_01305) pilN 279738..280322 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  HPY15_RS01310 (HPY15_01310) pilO 280315..280905 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY15_RS01315 (HPY15_01315) pilP 280895..281413 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  HPY15_RS01320 (HPY15_01320) pilQ 281439..283154 (+) 1716 WP_032470277.1 type IV pilus secretin PilQ Machinery gene
  HPY15_RS01325 (HPY15_01325) aroK 283341..283865 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37734.46 Da        Isoelectric Point: 7.3328

>NTDB_id=447449 HPY15_RS01300 WP_175235514.1 278732..279748(+) (pilM) [Vibrio cholerae strain SO5Y]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFNHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=447449 HPY15_RS01300 WP_175235514.1 278732..279748(+) (pilM) [Vibrio cholerae strain SO5Y]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAATCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCTGTTGAAGAGCTGAGCCTCGATTTCGTTAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGCTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGTGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485