Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   I5U72_RS26635 Genome accession   NZ_CP065417
Coordinates   5729321..5729746 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa isolate P23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5724321..5734746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5U72_RS26620 (I5U72_26620) pilX 5724885..5725472 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  I5U72_RS26625 (I5U72_26625) pilY1 5725484..5728975 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  I5U72_RS26630 (I5U72_26630) pilY2 5728977..5729324 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  I5U72_RS26635 (I5U72_26635) comF 5729321..5729746 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  I5U72_RS26640 (I5U72_26640) ispH 5729793..5730737 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  I5U72_RS26645 (I5U72_26645) fkpB 5730823..5731263 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  I5U72_RS26650 (I5U72_26650) lspA 5731256..5731765 (-) 510 WP_003110420.1 signal peptidase II -
  I5U72_RS26655 (I5U72_26655) ileS 5731758..5734589 (-) 2832 WP_023103515.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=446931 I5U72_RS26635 WP_003094721.1 5729321..5729746(+) (comF) [Pseudomonas aeruginosa isolate P23]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=446931 I5U72_RS26635 WP_003094721.1 5729321..5729746(+) (comF) [Pseudomonas aeruginosa isolate P23]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383