Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FX982_RS18665 Genome accession   NZ_CP053746
Coordinates   4167079..4168245 (+) Length   388 a.a.
NCBI ID   WP_172611987.1    Uniprot ID   A0A6M8MSN8
Organism   Pseudomonas graminis strain PgKB30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4162079..4173245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX982_RS18635 (FX982_03734) - 4162087..4162878 (+) 792 WP_108119374.1 amino acid ABC transporter ATP-binding protein -
  FX982_RS18640 (FX982_03735) - 4162903..4163289 (+) 387 WP_172611983.1 RidA family protein -
  FX982_RS18645 (FX982_03736) - 4163400..4164161 (+) 762 WP_172611984.1 IclR family transcriptional regulator -
  FX982_RS18650 (FX982_03737) - 4164207..4165412 (+) 1206 WP_172611985.1 amino acid deaminase -
  FX982_RS18655 (FX982_03738) - 4165425..4166366 (+) 942 WP_172611986.1 sugar kinase -
  FX982_RS18660 (FX982_03739) - 4166592..4166867 (-) 276 WP_037009819.1 peptidylprolyl isomerase -
  FX982_RS18665 (FX982_03740) pilU 4167079..4168245 (+) 1167 WP_172611987.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FX982_RS18670 (FX982_03741) - 4168435..4169205 (-) 771 WP_172611988.1 3-hydroxybutyrate dehydrogenase -
  FX982_RS18675 (FX982_03742) - 4169216..4170607 (-) 1392 WP_172611989.1 GntP family permease -
  FX982_RS18680 (FX982_03743) - 4170865..4172280 (-) 1416 WP_172611990.1 sigma-54-dependent Fis family transcriptional regulator -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 42829.88 Da        Isoelectric Point: 6.3929

>NTDB_id=446838 FX982_RS18665 WP_172611987.1 4167079..4168245(+) (pilU) [Pseudomonas graminis strain PgKB30]
MDFPALLKVLATQDGSDLYLSTGAPPCAKFNGVLKPLGTEALKPGDVAVIANSIMDAEQRVDFERELEMNLAFSLSGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLHLPPILLDVIMEKHGLVLFVGATGSGKSTSLAALIDHRNRHASGHIITIED
PVEFIHKHKRSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLHDLGNNLKAFVSQRLVKTTDGKRRAAVEVMLGTPTIRDLIHRNELTELKGIMEKSTNLGMQTFDNAL
YDLAVEGAITEDEALKNADSANNVRLRLKLHSEDGPSTIATAPRAPQPAAQVDVKDWGLVDERDEPGS

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=446838 FX982_RS18665 WP_172611987.1 4167079..4168245(+) (pilU) [Pseudomonas graminis strain PgKB30]
ATGGATTTTCCGGCGTTGTTGAAGGTGCTTGCCACCCAGGATGGATCGGACCTTTATCTGTCCACGGGCGCGCCCCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCACGGAAGCGCTCAAGCCCGGTGACGTCGCAGTGATTGCCAACAGCA
TCATGGACGCCGAGCAGCGCGTGGATTTCGAACGCGAGCTGGAAATGAACCTGGCCTTTTCCCTGTCTGGTGTCGGGCGT
TTCCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGATCGTGGCGCGCAACATCAAGCTCGACATCCCCAAATTCGA
AGACCTGCATCTGCCGCCGATCCTGCTCGACGTGATCATGGAGAAGCACGGTCTGGTGCTGTTCGTTGGCGCCACCGGTT
CAGGTAAGTCCACCTCGCTGGCGGCTTTGATCGATCATCGCAATCGCCACGCCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTTCATCCATAAGCACAAGCGATCGATCATCAATCAGCGCGAAGTGGGCGTCGACACCCGGAGCTTTCACGC
GGCCCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATTCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAACACG
CCTTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCAACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCCGAAGAGCGCCGCCCGCAGTTGCTGCATGATCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
GCTGGTCAAGACCACCGACGGCAAGCGCCGCGCGGCCGTGGAGGTGATGCTCGGCACGCCGACCATTCGCGACCTGATCC
ACCGCAATGAGCTGACCGAGCTCAAGGGCATCATGGAGAAGTCCACCAACCTCGGCATGCAGACCTTCGACAACGCGCTG
TATGACTTGGCGGTGGAGGGTGCGATCACCGAAGACGAGGCCTTGAAGAACGCCGACTCGGCCAACAACGTGCGCCTGCG
CTTGAAATTGCACAGCGAGGACGGGCCGTCGACCATCGCTACGGCGCCGCGCGCGCCCCAGCCGGCCGCGCAGGTGGACG
TCAAAGATTGGGGGCTGGTGGACGAGCGGGACGAGCCGGGGAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8MSN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.863

92.01

0.523
  pilU Acinetobacter baylyi ADP1

53.239

91.495

0.487
  pilU Vibrio cholerae strain A1552

52

90.206

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

87.371

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.108

88.402

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

85.825

0.361
  pilT Vibrio cholerae strain A1552

42.042

85.825

0.361