Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   I3251_RS11350 Genome accession   NZ_CP065374
Coordinates   2383050..2383475 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PAG7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2378050..2388475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3251_RS11335 (I3251_11335) pilX 2378614..2379201 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  I3251_RS11340 (I3251_11340) pilY1 2379213..2382704 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  I3251_RS11345 (I3251_11345) pilY2 2382706..2383053 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  I3251_RS11350 (I3251_11350) comF 2383050..2383475 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  I3251_RS11355 (I3251_11355) ispH 2383522..2384466 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  I3251_RS11360 (I3251_11360) fkpB 2384552..2384992 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  I3251_RS11365 (I3251_11365) lspA 2384985..2385494 (-) 510 WP_003102615.1 signal peptidase II -
  I3251_RS11370 (I3251_11370) ileS 2385487..2388318 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=446524 I3251_RS11350 WP_003094721.1 2383050..2383475(+) (comF) [Pseudomonas aeruginosa strain PAG7]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=446524 I3251_RS11350 WP_003094721.1 2383050..2383475(+) (comF) [Pseudomonas aeruginosa strain PAG7]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383