Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I3U01_RS20300 Genome accession   NZ_CP065203
Coordinates   4200051..4200518 (+) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain EC-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4195051..4205518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3U01_RS20255 (I3U01_20055) rplP 4195161..4195571 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  I3U01_RS20260 (I3U01_20060) rpsC 4195584..4196285 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  I3U01_RS20265 (I3U01_20065) rplV 4196303..4196635 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  I3U01_RS20270 (I3U01_20070) rpsS 4196650..4196928 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  I3U01_RS20275 (I3U01_20075) rplB 4196945..4197766 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  I3U01_RS20280 (I3U01_20080) rplW 4197784..4198086 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  I3U01_RS20285 (I3U01_20085) rplD 4198083..4198688 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  I3U01_RS20290 (I3U01_20090) rplC 4198699..4199328 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  I3U01_RS20295 (I3U01_20095) rpsJ 4199361..4199672 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  I3U01_RS20300 (I3U01_20100) pilD 4200051..4200518 (+) 468 WP_000340159.1 A24 family peptidase Machinery gene
  I3U01_RS20305 (I3U01_20105) bfr 4200515..4200991 (-) 477 WP_000675515.1 bacterioferritin -
  I3U01_RS20310 (I3U01_20110) bfd 4201064..4201258 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  I3U01_RS20315 (I3U01_20115) tuf 4201441..4202625 (-) 1185 WP_000031783.1 elongation factor Tu -
  I3U01_RS20320 (I3U01_20120) fusA 4202696..4204810 (-) 2115 WP_000124700.1 elongation factor G -
  I3U01_RS20325 (I3U01_20125) rpsG 4204907..4205377 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=446113 I3U01_RS20300 WP_000340159.1 4200051..4200518(+) (pilD) [Escherichia coli strain EC-10]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=446113 I3U01_RS20300 WP_000340159.1 4200051..4200518(+) (pilD) [Escherichia coli strain EC-10]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368