Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   I3J23_RS03695 Genome accession   NZ_CP065159
Coordinates   708808..711165 (-) Length   785 a.a.
NCBI ID   WP_015387880.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 703808..716165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS03670 (I3J23_03670) - 704311..705090 (-) 780 WP_003152548.1 enoyl-CoA hydratase -
  I3J23_RS03675 (I3J23_03675) - 705104..705688 (-) 585 WP_014470798.1 TetR/AcrR family transcriptional regulator -
  I3J23_RS03680 (I3J23_03680) - 705777..707465 (-) 1689 WP_003152545.1 AMP-binding protein -
  I3J23_RS03685 (I3J23_03685) - 707571..708248 (+) 678 WP_015387881.1 DUF2711 family protein -
  I3J23_RS03690 (I3J23_03690) - 708387..708791 (-) 405 WP_003152541.1 DUF350 domain-containing protein -
  I3J23_RS03695 (I3J23_03695) mutS/mutS2 708808..711165 (-) 2358 WP_015387880.1 endonuclease MutS2 Machinery gene
  I3J23_RS03700 (I3J23_03700) polX 711186..712898 (-) 1713 WP_046560235.1 DNA polymerase/3'-5' exonuclease PolX -
  I3J23_RS03705 (I3J23_03705) - 713004..713537 (-) 534 WP_003152534.1 CvpA family protein -
  I3J23_RS03710 (I3J23_03710) zapA 713545..713802 (-) 258 WP_043867333.1 cell division protein ZapA -
  I3J23_RS03715 (I3J23_03715) rnhC 713936..714877 (+) 942 WP_003152532.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87318.66 Da        Isoelectric Point: 6.4184

>NTDB_id=445761 I3J23_RS03695 WP_015387880.1 708808..711165(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain GXU-1]
MQQKVLSSLEFHKVKEQITAHAASSLGREKLLQLKPLTDLADIQKQLDEVEEASAVMRLRGHAPFGGLTDIRSALRRAEI
GSVLTPAEFTELSGLLYAVKQMKHFISQMTEDGVGIPLIQAHAEELITLGDLEREINSCIDDHGEVLDHASPALRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRMLTEHTAEHTQEIAQNVEVLQTLDFIFAKARYAKAMKATKPFMNGDGFIRLKKARHPLLPQDQVVAN
DIELGGDYSTIVITGPNTGGKTVTLKTLGLLTIMAQAGLHIPADEGSEAAVFDNVFADIGDEQSIEQSLSTFSSHMVNIV
NILKDVSENSLVLFDELGAGTDPQEGAALAMSILDEVHRTNARVLATTHYPELKAYGYNRQGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISRRLGLPEHIIGQAKSEMTAEHNEVDLMIASLEKSKKRADEELSETESLRKEAEKLHKELQQQIIE
LNAQKDKMMEEAERKAAEKLEAAANEAEQIIRELRSIKQEHRSFKEHELIDAKKRLGDAMPAFEKSKQPERKTEKKRELK
PGDEVKVLTFGQKGALLEKTGEKEWNVQIGILKMKVKEKDLEFLKSAPEPKKEKAITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTIVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=445761 I3J23_RS03695 WP_015387880.1 708808..711165(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain GXU-1]
GTGCAGCAAAAAGTGTTATCATCACTTGAATTTCATAAAGTAAAAGAACAGATTACCGCACATGCCGCCTCATCGCTCGG
CAGGGAGAAGCTTCTGCAGCTGAAACCTTTGACGGATCTCGCCGACATCCAAAAACAGCTGGATGAAGTCGAAGAAGCAT
CCGCGGTCATGCGCTTGAGAGGCCATGCTCCGTTCGGCGGCCTGACCGACATAAGATCCGCTTTACGGCGGGCGGAAATC
GGCAGTGTGCTGACGCCGGCGGAATTTACCGAATTGTCCGGGCTGCTGTATGCCGTTAAACAAATGAAGCATTTTATCAG
CCAAATGACAGAAGACGGCGTCGGCATTCCGCTGATACAGGCCCATGCAGAAGAGCTGATTACATTAGGCGATTTGGAAA
GGGAGATTAACTCCTGCATTGATGACCATGGTGAAGTGCTTGATCATGCGTCTCCCGCTTTAAGGGGAATCCGCACGCAG
CTCAGGACGCTTGAATCAAGAGTCAGAGACCGGCTTGAATCGATGCTGCGTTCATCTTCCGCTTCTAAAATGCTGTCTGA
CACCATCGTTACGATTCGGAATGACCGCTTTGTCATCCCGGTCAAACAGGAATACAGATCAAGCTACGGCGGAATTGTCC
ACGATACATCTTCATCCGGTGCGACACTGTTTATTGAACCGCAGGCGATCGTAGATATGAACAACTCGCTTCAGCAGGCG
AAAGTAAAAGAAAAACAGGAAATCGAACGGATTCTCCGGATGCTGACGGAGCATACGGCTGAACACACACAAGAAATCGC
TCAAAATGTGGAAGTGCTGCAGACACTGGATTTCATTTTCGCCAAAGCGAGATACGCAAAAGCAATGAAGGCGACAAAAC
CATTCATGAACGGAGACGGCTTTATCCGTCTGAAAAAAGCGCGCCATCCGCTGCTGCCCCAAGATCAAGTCGTGGCCAAC
GATATTGAACTGGGCGGAGATTATTCAACGATCGTCATCACGGGACCGAATACGGGCGGTAAAACCGTTACGTTAAAAAC
ACTCGGGCTGCTGACGATAATGGCGCAGGCAGGATTGCACATACCGGCTGATGAGGGATCGGAAGCAGCCGTATTTGACA
ATGTGTTTGCCGATATCGGCGATGAACAGTCGATCGAACAAAGTCTGAGTACGTTCTCATCCCACATGGTCAATATCGTC
AATATTTTAAAAGACGTGTCTGAAAACAGTCTTGTGCTGTTCGATGAACTCGGTGCCGGAACAGATCCGCAGGAAGGGGC
TGCGCTTGCCATGAGCATCCTTGACGAAGTTCACCGGACAAACGCCAGAGTGCTTGCGACAACCCATTATCCGGAACTGA
AAGCATACGGATATAACAGACAAGGCGTCATGAATGCCAGCGTTGAATTTGACATTGAAACGCTTTCGCCTACCTACAAG
CTTCTGATCGGAGTGCCGGGCCGAAGCAACGCATTTGAAATCTCAAGACGCCTTGGGCTTCCGGAGCATATCATCGGCCA
GGCGAAGTCAGAAATGACCGCCGAACATAACGAAGTCGATCTGATGATCGCATCGCTTGAAAAAAGCAAAAAAAGAGCGG
ATGAAGAGCTTTCTGAAACCGAATCACTCAGAAAAGAAGCAGAAAAACTGCACAAAGAGCTTCAGCAGCAAATCATTGAG
CTGAACGCCCAAAAAGATAAAATGATGGAAGAAGCCGAGCGGAAAGCTGCGGAAAAACTGGAAGCCGCCGCAAATGAAGC
CGAACAGATTATCCGCGAGCTCAGGTCCATCAAGCAAGAACACAGATCCTTTAAGGAACACGAGCTGATTGACGCCAAAA
AACGTCTCGGAGACGCGATGCCGGCTTTTGAAAAATCAAAGCAGCCGGAAAGAAAAACAGAGAAAAAACGCGAGCTGAAG
CCGGGCGACGAAGTAAAGGTGCTCACATTCGGACAAAAAGGGGCGCTGCTTGAAAAAACCGGCGAGAAAGAATGGAACGT
CCAAATCGGCATCCTTAAAATGAAGGTGAAGGAAAAAGATCTTGAGTTTCTCAAATCGGCTCCTGAACCGAAAAAAGAAA
AAGCAATCACCGCCGTAAAAGGGAAGGATTATCACGTATCCCTTGAACTTGACCTCAGGGGAGAGCGTTACGAAAACGCC
CTCAGCCGCGTCGAAAAATATTTAGATGACGCGGTGCTCGCCGGCTATCCGAGAGTGTCCATCATTCACGGAAAAGGCAC
GGGCGCCCTCCGAAAAGGGGTGCAGGATCTTCTGAAAAACCACCGCAGCGTGAAAAGCTCGCGGTTCGGGGAAGCGGGAG
AAGGAGGATCAGGCGTAACGATTGTCGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS domain V (MutS_V)

(331-512)

Smr domain (Smr)

(710-785)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

87.389

100

0.874