Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IPT86_RS21480 Genome accession   NZ_CP064847
Coordinates   4437352..4437819 (+) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain 20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4432352..4442819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IPT86_RS21435 (IPT86_21435) rplP 4432462..4432872 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  IPT86_RS21440 (IPT86_21440) rpsC 4432885..4433586 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  IPT86_RS21445 (IPT86_21445) rplV 4433604..4433936 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  IPT86_RS21450 (IPT86_21450) rpsS 4433951..4434229 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  IPT86_RS21455 (IPT86_21455) rplB 4434246..4435067 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  IPT86_RS21460 (IPT86_21460) rplW 4435085..4435387 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  IPT86_RS21465 (IPT86_21465) rplD 4435384..4435989 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  IPT86_RS21470 (IPT86_21470) rplC 4436000..4436629 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  IPT86_RS21475 (IPT86_21475) rpsJ 4436662..4436973 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  IPT86_RS21480 (IPT86_21480) pilD 4437352..4437819 (+) 468 WP_000340159.1 A24 family peptidase Machinery gene
  IPT86_RS21485 (IPT86_21485) bfr 4437816..4438292 (-) 477 WP_000675515.1 bacterioferritin -
  IPT86_RS21490 (IPT86_21490) bfd 4438365..4438559 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  IPT86_RS21495 (IPT86_21495) tuf 4438742..4439926 (-) 1185 WP_000031783.1 elongation factor Tu -
  IPT86_RS21500 (IPT86_21500) fusA 4439997..4442111 (-) 2115 WP_000124700.1 elongation factor G -
  IPT86_RS21505 (IPT86_21505) rpsG 4442208..4442678 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=445318 IPT86_RS21480 WP_000340159.1 4437352..4437819(+) (pilD) [Escherichia coli strain 20]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=445318 IPT86_RS21480 WP_000340159.1 4437352..4437819(+) (pilD) [Escherichia coli strain 20]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368