Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   IUJ43_RS18645 Genome accession   NZ_CP064391
Coordinates   4053662..4054087 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4048662..4059087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ43_RS18630 (IUJ43_18590) pilX 4049232..4049819 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  IUJ43_RS18635 (IUJ43_18595) pilY1 4049831..4053316 (+) 3486 WP_003115287.1 type 4a pilus biogenesis protein PilY1 -
  IUJ43_RS18640 (IUJ43_18600) pilY2 4053318..4053665 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  IUJ43_RS18645 (IUJ43_18605) comF 4053662..4054087 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  IUJ43_RS18650 (IUJ43_18610) ispH 4054134..4055078 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  IUJ43_RS18655 (IUJ43_18615) fkpB 4055164..4055604 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  IUJ43_RS18660 (IUJ43_18620) lspA 4055597..4056106 (-) 510 WP_003112823.1 signal peptidase II -
  IUJ43_RS18665 (IUJ43_18625) ileS 4056099..4058930 (-) 2832 WP_003112822.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=443676 IUJ43_RS18645 WP_003094721.1 4053662..4054087(+) (comF) [Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=443676 IUJ43_RS18645 WP_003094721.1 4053662..4054087(+) (comF) [Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383