Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SYNGTI_RS12070 Genome accession   NC_017038
Coordinates   2587569..2589587 (+) Length   672 a.a.
NCBI ID   WP_010873519.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803 substr. GT-I     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2582569..2594587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SYNGTI_RS12055 (SYNGTI_2325) - 2583207..2584241 (-) 1035 WP_010873516.1 AI-2E family transporter -
  SYNGTI_RS12060 (SYNGTI_2326) dnaK 2584290..2586368 (-) 2079 WP_010873517.1 molecular chaperone DnaK -
  SYNGTI_RS12065 (SYNGTI_2327) grpE 2586602..2587351 (-) 750 WP_010873518.1 nucleotide exchange factor GrpE -
  SYNGTI_RS12070 (SYNGTI_2328) pilB/pilB1 2587569..2589587 (+) 2019 WP_010873519.1 GspE/PulE family protein Machinery gene
  SYNGTI_RS12075 (SYNGTI_2329) - 2589671..2590825 (+) 1155 WP_010873520.1 RNA methyltransferase -
  SYNGTI_RS12080 (SYNGTI_2330) - 2591047..2591685 (+) 639 WP_010873521.1 class I SAM-dependent methyltransferase -
  SYNGTI_RS12085 (SYNGTI_2331) ribD 2591773..2592879 (+) 1107 WP_010873522.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
  SYNGTI_RS16980 - 2592830..2592982 (-) 153 WP_071822661.1 DUF2256 domain-containing protein -
  SYNGTI_RS12090 (SYNGTI_2332) - 2592998..2594284 (-) 1287 WP_010873523.1 pitrilysin family protein -

Sequence

Protein


Download         Length: 672 a.a.        Molecular weight: 75111.64 Da        Isoelectric Point: 5.2598

>NTDB_id=44344 SYNGTI_RS12070 WP_010873519.1 2587569..2589587(+) (pilB/pilB1) [Synechocystis sp. PCC 6803 substr. GT-I]
MTSSSSSKKSRAVALRNYFSPFGNKLVAGGHVDAEQLRQALVQVKKTGRSLPEELKAVTGRELSPELLRQYKKNQLFELK
VLYGVDSVDPEVAPIATQQMAELIGRFFPLDTCRRFKFLPLAQQEGDPPSVLVAMVDPDNLAAQDELNRILRVKGFELRR
LVVTQDDFNQLLEQYYAIREEWEAAQEKRDQERALDKLNDLTDIVGSIDMNLGEDKEDVDNALDSNDANQAPVINLVNKI
LAKALQEGTSDIHVEPQEETLRIRFRKDGVLNQAFDPLPRKITPAVVARFKIMADMDIAERRSPQDGKIRRIYQGRKVDF
RVNTLPSRYGEKVCLRILDNSATQLGLDFLITNPETLQTVRELAARPYGLMLVTGPTGSGKSTTLYSVLAERNSPEVNIN
TAEDPIEYALPGITQVQVIREKGMDFSNILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIAR
LDEMGVEPFMVSGSLLGVLAQRLMRKVCTECRIAYHPSKEELSRFGLSASGDEVVTFYKANSLSPDEIHLARQKGNLCQK
CSGSGYKGRVGVYEVMRNSERIAELINQGATTDRIKDCAVEEGMITLLAYSLNLVQEGYTTLEEVERVTFTDTGLESEMR
AKRKSALTCRTCAAELQQEWLDCPYCMTSRFS

Nucleotide


Download         Length: 2019 bp        

>NTDB_id=44344 SYNGTI_RS12070 WP_010873519.1 2587569..2589587(+) (pilB/pilB1) [Synechocystis sp. PCC 6803 substr. GT-I]
ATGACATCTTCCTCCTCTTCCAAGAAAAGCCGTGCCGTTGCCCTACGAAATTATTTTTCCCCCTTCGGCAATAAATTGGT
GGCGGGGGGCCATGTAGATGCTGAACAATTGCGACAGGCCCTAGTCCAGGTGAAAAAAACTGGGCGGTCTTTGCCCGAGG
AACTTAAGGCAGTCACTGGCCGAGAATTGTCCCCAGAACTACTGCGGCAGTACAAAAAAAATCAGTTATTTGAGCTCAAA
GTACTTTACGGGGTGGACAGCGTCGATCCGGAGGTTGCCCCGATCGCCACCCAGCAAATGGCCGAGTTGATTGGGCGATT
TTTTCCTTTGGATACTTGTCGGCGATTCAAGTTTTTGCCCTTAGCCCAGCAGGAAGGCGACCCCCCCAGTGTGCTAGTGG
CCATGGTGGACCCCGATAACTTGGCTGCCCAGGACGAGCTAAACCGAATTTTGCGGGTTAAAGGTTTTGAATTGCGCCGC
TTGGTGGTCACCCAAGATGATTTTAACCAACTGTTGGAACAGTACTATGCCATCCGGGAAGAGTGGGAGGCGGCCCAGGA
AAAAAGGGACCAGGAAAGGGCCCTTGATAAACTTAATGATTTGACGGATATTGTCGGCTCCATCGACATGAATCTGGGGG
AGGACAAAGAAGATGTAGATAATGCTCTAGACTCCAACGATGCTAACCAAGCACCGGTGATTAACCTGGTCAACAAAATT
TTGGCTAAGGCTCTCCAGGAAGGAACTTCCGACATTCACGTGGAGCCGCAGGAAGAAACCCTGAGAATTCGTTTCCGCAA
GGATGGGGTTTTAAATCAGGCTTTCGATCCCCTGCCCCGCAAAATTACCCCCGCCGTGGTGGCCCGTTTTAAAATTATGG
CGGACATGGACATTGCGGAGAGGCGATCGCCCCAGGATGGCAAAATTCGCCGTATTTACCAGGGCAGGAAGGTGGATTTT
CGGGTTAATACCCTACCCAGCCGCTATGGGGAAAAGGTTTGTTTGCGGATTTTGGACAACTCCGCCACTCAGTTGGGCTT
AGATTTTCTAATCACTAACCCCGAAACCCTACAAACGGTAAGGGAATTGGCCGCCCGGCCCTATGGCTTAATGTTGGTGA
CGGGGCCCACCGGATCGGGAAAATCCACCACCCTCTACTCGGTGTTGGCGGAACGCAATAGCCCCGAGGTGAATATCAAC
ACAGCGGAGGACCCGATTGAGTATGCTCTACCGGGCATTACCCAAGTGCAGGTGATTCGGGAAAAGGGCATGGATTTTTC
CAATATTCTGCGGGCATTTATGCGTCAGGATCCCGATGTGATTCTGGTGGGGGAAACCAGGGATAAGGAGACGGCCAAAA
CGGCCATTGAGGCGGCTCTGACTGGACACTTAGTTCTCACTACTCTCCACACCAATGATGCGGCCGGGGCGATCGCCAGA
TTGGATGAAATGGGAGTGGAGCCGTTTATGGTTTCTGGGTCATTGTTGGGGGTGTTGGCCCAACGGCTGATGCGGAAAGT
TTGTACGGAATGTCGCATTGCTTACCACCCTAGCAAGGAAGAATTGTCCCGCTTTGGGCTGTCCGCTTCCGGGGATGAAG
TGGTTACTTTTTATAAAGCCAATTCCCTCAGTCCCGACGAAATCCATCTTGCCCGTCAAAAAGGTAATTTATGTCAAAAA
TGTAGTGGCAGTGGCTATAAGGGTCGGGTGGGGGTCTATGAGGTGATGCGTAATTCTGAGCGTATTGCTGAATTGATTAA
CCAGGGGGCCACCACTGACCGCATTAAGGATTGTGCGGTGGAAGAGGGCATGATCACCCTATTGGCCTATAGTCTTAATT
TAGTGCAGGAAGGCTACACCACCCTAGAGGAGGTGGAACGGGTCACTTTCACCGATACCGGACTGGAGTCAGAAATGCGG
GCTAAGCGCAAGAGTGCTTTAACCTGTCGTACGTGTGCGGCGGAACTGCAACAGGAATGGCTTGATTGTCCCTATTGCAT
GACTTCCCGGTTTAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(73-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

100

100

1

Multiple sequence alignment