Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IQS23_RS19810 Genome accession   NZ_CP063476
Coordinates   4152228..4152695 (-) Length   155 a.a.
NCBI ID   WP_022646190.1    Uniprot ID   -
Organism   Escherichia coli strain EM10-18-28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4147228..4157695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS23_RS19785 (IQS23_19790) rpsG 4147369..4147839 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  IQS23_RS19790 (IQS23_19795) fusA 4147936..4150050 (+) 2115 WP_000124700.1 elongation factor G -
  IQS23_RS19795 (IQS23_19800) tuf 4150121..4151305 (+) 1185 WP_000031783.1 elongation factor Tu -
  IQS23_RS19800 (IQS23_19805) bfd 4151488..4151682 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  IQS23_RS19805 (IQS23_19810) bfr 4151755..4152231 (+) 477 WP_000675515.1 bacterioferritin -
  IQS23_RS19810 (IQS23_19815) pilD 4152228..4152695 (-) 468 WP_022646190.1 A24 family peptidase Machinery gene
  IQS23_RS19815 (IQS23_19820) rpsJ 4153074..4153385 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  IQS23_RS19820 (IQS23_19825) rplC 4153418..4154047 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  IQS23_RS19825 (IQS23_19830) rplD 4154058..4154663 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  IQS23_RS19830 (IQS23_19835) rplW 4154660..4154962 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  IQS23_RS19835 (IQS23_19840) rplB 4154980..4155801 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  IQS23_RS19840 (IQS23_19845) rpsS 4155818..4156096 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  IQS23_RS19845 (IQS23_19850) rplV 4156111..4156443 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  IQS23_RS19850 (IQS23_19855) rpsC 4156461..4157162 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  IQS23_RS19855 (IQS23_19860) rplP 4157175..4157585 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17064.20 Da        Isoelectric Point: 8.1255

>NTDB_id=439844 IQS23_RS19810 WP_022646190.1 4152228..4152695(-) (pilD) [Escherichia coli strain EM10-18-28]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLATAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=439844 IQS23_RS19810 WP_022646190.1 4152228..4152695(-) (pilD) [Escherichia coli strain EM10-18-28]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATTCTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGACTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368