Detailed information    

experimental Experimentally validated

Overview

Name   psaA   Type   Unclear
Locus tag   SPR_RS07420 Genome accession   NC_003098
Coordinates   1470686..1471615 (+) Length   309 a.a.
NCBI ID   WP_000733056.1    Uniprot ID   D2CTJ2
Organism   Streptococcus pneumoniae R6     
Function   require for competence; Mn permease   
Unclear

Function

The adcCBA putative operon of Streptococcus pneumoniae, an important human pathogen, was identified in a search for transformation-deficient mutants. The addition of Zn also restored near-normal spontaneous transformation of adc mutant cells in standard transformation medium. The removal of Mn from standard transformation medium also resulted in transformation deficiency of psaA mutant cells.


Genomic Context

Location: 1465686..1476615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS07400 (spr1490) - 1466152..1466781 (+) 630 WP_000690846.1 MBL fold metallo-hydrolase -
  SPR_RS07405 (spr1491) pepO 1466904..1468796 (-) 1893 WP_000199272.1 endopeptidase PepO -
  SPR_RS07410 (spr1492) - 1469087..1469809 (+) 723 WP_000619138.1 metal ABC transporter ATP-binding protein -
  SPR_RS07415 (spr1493) - 1469806..1470654 (+) 849 WP_000559782.1 metal ABC transporter permease -
  SPR_RS07420 (spr1494) psaA 1470686..1471615 (+) 930 WP_000733056.1 metal ABC transporter substrate-binding lipoprotein/adhesin PsaA Unclear
  SPR_RS07425 (spr1495) tpx 1471738..1472229 (+) 492 WP_000256028.1 thiol peroxidase -
  SPR_RS07430 (spr1496) - 1472325..1475033 (-) 2709 WP_000595871.1 ABC transporter permease -
  SPR_RS07435 (spr1497) - 1475035..1475736 (-) 702 WP_000323372.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 309 a.a.        Molecular weight: 34594.34 Da        Isoelectric Point: 5.0617

>NTDB_id=439 SPR_RS07420 WP_000733056.1 1470686..1471615(+) (psaA) [Streptococcus pneumoniae R6]
MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIADITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTS
EADLIFYNGINLETGGNAWFTKLVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEINTEEEGTPEQIKTLV
EKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK

Nucleotide


Download         Length: 930 bp        

>NTDB_id=439 SPR_RS07420 WP_000733056.1 1470686..1471615(+) (psaA) [Streptococcus pneumoniae R6]
ATGAAAAAATTAGGTACATTACTCGTTCTCTTTCTTTCTGCAATCATTCTTGTAGCATGTGCTAGCGGAAAAAAAGATAC
AACTTCTGGTCAAAAACTAAAAGTTGTTGCTACAAACTCAATCATCGCTGATATTACTAAAAATATTGCTGGTGACAAAA
TTGACCTTCATAGTATCGTTCCGATTGGGCAAGACCCACACGAATACGAACCACTTCCTGAAGACGTTAAGAAAACTTCT
GAGGCTGATTTGATTTTCTATAACGGTATCAACCTTGAAACAGGTGGCAATGCTTGGTTTACAAAATTGGTAGAAAATGC
CAAGAAAACTGAAAACAAAGACTACTTCGCAGTCAGCGACGGCGTTGATGTTATCTACCTTGAAGGTCAAAATGAAAAAG
GAAAAGAAGACCCACACGCTTGGCTTAACCTTGAAAACGGTATTATTTTTGCTAAAAATATCGCCAAACAATTGAGCGCC
AAAGACCCTAACAATAAAGAATTCTATGAAAAAAATCTCAAAGAATATACTGATAAGTTAGACAAACTTGATAAAGAAAG
TAAGGATAAATTTAATAAGATCCCTGCTGAAAAGAAACTCATTGTAACCAGCGAAGGAGCATTCAAATACTTCTCTAAAG
CCTATGGTGTTCCAAGTGCCTACATCTGGGAAATCAATACTGAAGAAGAAGGAACTCCTGAACAAATCAAGACCTTGGTT
GAAAAACTTCGCCAAACAAAAGTTCCATCACTCTTTGTAGAATCAAGTGTGGATGACCGTCCAATGAAAACTGTTTCTCA
AGACACAAACATCCCAATCTACGCACAAATCTTTACTGACTCTATCGCAGAACAAGGTAAAGAAGGCGACAGCTACTACA
GCATGATGAAATACAACCTTGACAAGATTGCTGAAGGATTGGCAAAATAA

Domains

Predicted by InterproScan.

Zinc-uptake complex component A periplasmic (ZnuA)

(35-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2CTJ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] A Dintilhac et al. (1997) Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases. Molecular Microbiology 25(4):727-39. [PMID: 9379902]