Detailed information NTG0436921

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HGD76_RS10830
• Genome accession: NZ_CP051206
• Coordinates: 2189382..2190488 (+)
• Length: 368 a.a.
• NCBI ID: WP_148761666.1
• Uniprot ID: A0A6H2C058
• Organism: Dolichospermum flos-aquae CCAP 1403/13F
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2184382..2195488

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HGD76_RS10800 (HGD76_10805)	-	2184470..2184985 (-)	516	WP_168507414.1	TIGR02652 family protein	-
HGD76_RS10805 (HGD76_10810)	-	2185225..2185728 (+)	504	WP_041457827.1	gamma carbonic anhydrase family protein	-
HGD76_RS10810 (HGD76_10815)	-	2185915..2186040 (+)	126	WP_148761662.1	photosystem II protein Y	-
HGD76_RS10815 (HGD76_10820)	-	2186126..2187379 (+)	1254	WP_168695780.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-
HGD76_RS10820 (HGD76_10825)	-	2187570..2188868 (-)	1299	WP_168695781.1	sugar ABC transporter substrate-binding protein	-
HGD76_RS25335	-	2188895..2189188 (-)	294	WP_233467176.1	hypothetical protein	-
HGD76_RS25340	-	2189133..2189276 (-)	144	WP_233467177.1	hypothetical protein	-
HGD76_RS10830 (HGD76_10835)	pilM	2189382..2190488 (+)	1107	WP_148761666.1	type IV pilus assembly protein PilM	Machinery gene
HGD76_RS10835 (HGD76_10840)	-	2190493..2191302 (+)	810	WP_168695782.1	PilN domain-containing protein	-
HGD76_RS10840 (HGD76_10845)	-	2191299..2192066 (+)	768	WP_168695783.1	pilus assembly protein PilO	-
HGD76_RS10845 (HGD76_10850)	-	2192180..2194468 (+)	2289	WP_168695784.1	type IV pilus secretin family protein	-
HGD76_RS10850 (HGD76_10855)	pyrR	2194598..2195134 (-)	537	WP_148761674.1	bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 40501.52 Da
• Isoelectric Point: 4.4409

>NTDB_id=436921 HGD76_RS10830 WP_148761666.1 2189382..2190488(+) (pilM) [Dolichospermum flos-aquae CCAP 1403/13F]
MVKAFTSFFGKSSKGIGIELAPERVNVVQLHKHRQNLKIESLTTVPVPEGIFIDGQIADSVGMSEIIQQAIAESKTKATR
VITCIPGRESIIRLIPVPSELDDKELRDIILNHEASLYLPYPREEADVDYQKLGDFIDEDGIEKVQVLLVATRKDVTQTY
LNTFEQAGLTVDILEINSFALIRTIRDQLRLFGPEEAAVLVDIEFDSTEIAIIINGIPQFSRTVPIGTYQLQTALSRAMN
LPASRDMDMLESMTIPTTSPDSAHSGVTGSNPGMASMLRVLGELADELRRSIDFYLNQSENLEVVQILLAGPGGGLPQID
EFFTQRLSLPTTQIDPITSLSLEVDAEKYPLIKRPGLGIVLGLGMRGL

Nucleotide

• Length: 1107 bp

>NTDB_id=436921 HGD76_RS10830 WP_148761666.1 2189382..2190488(+) (pilM) [Dolichospermum flos-aquae CCAP 1403/13F]
ATGGTTAAAGCCTTCACTAGTTTCTTTGGTAAATCCAGTAAAGGTATTGGTATTGAACTAGCGCCGGAACGGGTAAACGT
AGTCCAACTACATAAACACCGCCAAAACTTAAAAATCGAATCTTTGACAACAGTACCAGTACCAGAAGGTATATTTATTG
ATGGTCAAATTGCTGATTCTGTGGGAATGTCCGAAATTATTCAGCAAGCGATCGCTGAAAGCAAAACTAAAGCTACCCGT
GTTATTACTTGTATCCCAGGACGAGAATCCATTATCCGGTTGATTCCTGTCCCATCTGAATTAGATGACAAGGAGTTGCG
GGACATAATACTCAACCATGAAGCATCTTTATATTTACCCTATCCCAGAGAAGAAGCTGATGTTGATTATCAAAAACTTG
GGGACTTTATAGACGAAGACGGCATTGAGAAAGTGCAGGTTTTGTTAGTTGCGACTCGTAAAGATGTCACGCAAACCTAT
TTAAATACCTTTGAACAGGCAGGATTAACAGTTGATATCTTAGAAATTAACAGCTTTGCCCTGATTCGCACTATTCGTGA
TCAATTGCGGCTATTTGGACCAGAAGAAGCAGCGGTATTAGTTGATATTGAGTTTGATAGTACAGAAATAGCCATTATTA
TCAATGGTATCCCACAATTTTCTCGAACAGTACCGATTGGCACTTATCAACTCCAAACTGCATTGTCAAGGGCTATGAAC
TTACCAGCATCACGGGATATGGATATGTTGGAGAGTATGACTATTCCCACAACTTCACCAGATTCAGCCCATAGTGGAGT
GACAGGTAGTAATCCGGGGATGGCTTCTATGTTGAGAGTTCTCGGAGAATTAGCTGATGAATTGCGTCGTTCCATTGATT
TTTACCTCAATCAAAGTGAAAATCTGGAAGTCGTGCAGATTCTTTTAGCCGGTCCTGGGGGAGGATTACCACAAATTGAT
GAGTTTTTCACTCAAAGATTGAGTTTACCAACTACCCAAATAGATCCAATTACTTCTTTGTCTTTGGAGGTAGATGCTGA
AAAATATCCTTTAATTAAACGTCCTGGGTTGGGTATTGTCCTGGGTTTAGGAATGCGAGGGTTGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-367)

3D structure

• AlphaFold DB: A0A6H2C058

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Synechocystis sp. PCC 6803	63.934	99.457	0.636