Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HCU65_RS12800 Genome accession   NZ_CP050705
Coordinates   2592455..2593492 (-) Length   345 a.a.
NCBI ID   WP_061669999.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain PENSV20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2587455..2598492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU65_RS12750 (HCU65_12780) tasA 2587612..2588394 (-) 783 WP_003325439.1 biofilm matrix protein TasA -
  HCU65_RS12755 (HCU65_12785) sipW 2588458..2589030 (-) 573 WP_010789195.1 signal peptidase I SipW -
  HCU65_RS12760 (HCU65_12790) tapA 2589014..2589715 (-) 702 WP_219946339.1 amyloid fiber anchoring/assembly protein TapA -
  HCU65_RS12765 (HCU65_12795) - 2589977..2590300 (+) 324 WP_003325436.1 DUF3889 domain-containing protein -
  HCU65_RS12770 (HCU65_12800) - 2590347..2590526 (-) 180 WP_003325435.1 YqzE family protein -
  HCU65_RS12775 (HCU65_12805) comGG 2590596..2590970 (-) 375 WP_219946340.1 competence type IV pilus minor pilin ComGG Machinery gene
  HCU65_RS12780 (HCU65_12810) comGF 2590971..2591477 (-) 507 WP_309143169.1 competence type IV pilus minor pilin ComGF -
  HCU65_RS12785 (HCU65_12815) comGE 2591380..2591727 (-) 348 WP_003325432.1 competence type IV pilus minor pilin ComGE Machinery gene
  HCU65_RS12790 (HCU65_12820) comGD 2591711..2592151 (-) 441 WP_088117571.1 competence type IV pilus minor pilin ComGD Machinery gene
  HCU65_RS12795 (HCU65_12825) comGC 2592141..2592440 (-) 300 WP_003325429.1 competence type IV pilus major pilin ComGC Machinery gene
  HCU65_RS12800 (HCU65_12830) comGB 2592455..2593492 (-) 1038 WP_061669999.1 competence type IV pilus assembly protein ComGB Machinery gene
  HCU65_RS12805 (HCU65_12835) comGA 2593479..2594549 (-) 1071 WP_061669998.1 competence type IV pilus ATPase ComGA Machinery gene
  HCU65_RS12810 (HCU65_12840) - 2594945..2595898 (-) 954 WP_003325425.1 magnesium transporter CorA family protein -
  HCU65_RS12815 (HCU65_12845) - 2596020..2597368 (+) 1349 Protein_2522 hemolysin family protein -
  HCU65_RS12820 (HCU65_12850) - 2597434..2598285 (-) 852 WP_219946343.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 40112.34 Da        Isoelectric Point: 9.7517

>NTDB_id=434131 HCU65_RS12800 WP_061669999.1 2592455..2593492(-) (comGB) [Bacillus atrophaeus strain PENSV20]
MKRIKKSWPINDQANLLNRLGDMTENGYNLLDGLRLMELQMNKRQRTEMSAAVNRLREGEPFYQVLQALAFHKEAVSICY
LAEMHGELSVAMRQSGGLLERKLTQANQLKKVIRYPIFLIFTVFVMFYILQAVIIPQFSGVYQSMNMETSRTTAVIFAFF
QHVHYAVIGFVLLALGAALYYWFGFKKKSPADKMLFFVRIPILGRLVILFNSYFFSLQLSSLLKSGLSIYDSLKAFKEQS
FLPFYRQEAEQIINKLRAGESIDRALYKHPCYENDFCEVVLHGQLSGQLHRELFTYSQFLMERLERKVEKWTGILQPVIY
GIVAGMILIVYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=434131 HCU65_RS12800 WP_061669999.1 2592455..2593492(-) (comGB) [Bacillus atrophaeus strain PENSV20]
ATGAAGAGGATTAAAAAAAGCTGGCCGATAAACGATCAAGCTAATTTGCTAAACAGACTCGGTGACATGACAGAAAACGG
ATACAATCTTCTGGATGGATTGCGGTTAATGGAACTGCAAATGAATAAGCGTCAGCGTACGGAAATGTCTGCTGCTGTAA
ATCGTTTACGAGAGGGGGAACCATTCTATCAGGTTTTGCAGGCTTTGGCTTTCCACAAGGAAGCCGTAAGCATTTGTTAT
TTAGCAGAAATGCATGGAGAATTGTCCGTTGCTATGAGGCAAAGCGGAGGGCTGTTGGAAAGGAAATTAACACAGGCCAA
TCAGCTGAAAAAGGTCATCCGTTATCCGATTTTCCTGATCTTCACTGTCTTTGTGATGTTTTATATCCTGCAAGCTGTCA
TTATCCCGCAATTTTCAGGAGTTTATCAGTCCATGAATATGGAAACCTCACGCACAACCGCTGTTATATTCGCTTTTTTT
CAGCACGTTCATTATGCAGTGATTGGTTTTGTCTTGCTTGCTCTCGGAGCGGCACTCTATTACTGGTTTGGATTTAAGAA
GAAGTCCCCGGCAGACAAAATGCTCTTTTTTGTACGAATACCCATACTCGGAAGACTTGTCATTTTATTTAACAGCTACT
TTTTTTCTTTGCAGCTCAGCAGCCTATTAAAGTCTGGACTGTCTATTTACGACAGCCTTAAGGCCTTTAAAGAGCAATCG
TTCCTCCCTTTTTACCGTCAGGAGGCAGAACAAATCATTAACAAATTAAGGGCTGGAGAGTCCATAGATAGAGCTCTGTA
TAAACACCCATGCTATGAAAATGACTTTTGTGAAGTGGTGCTGCACGGCCAATTAAGCGGGCAGCTGCATCGTGAACTAT
TCACCTACAGCCAGTTTTTAATGGAGCGGCTGGAGCGAAAGGTTGAAAAATGGACGGGAATCCTTCAGCCCGTCATCTAC
GGTATTGTTGCCGGAATGATTTTAATTGTGTATTTGTCGATGCTTTTGCCTATGTATCAGATGATGAATCAATTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

69.35

93.623

0.649