Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   HBA75_RS05615 Genome accession   NZ_CP050183
Coordinates   1091401..1094916 (+) Length   1171 a.a.
NCBI ID   WP_086412620.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain HER1410     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1086401..1099916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA75_RS05575 (HBA75_05580) - 1086569..1087063 (-) 495 WP_000182396.1 DUF6584 family protein -
  HBA75_RS05580 (HBA75_05585) cspA 1087347..1087550 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  HBA75_RS05585 (HBA75_05590) - 1087748..1087951 (-) 204 WP_000356924.1 hypothetical protein -
  HBA75_RS05590 (HBA75_05595) - 1088297..1088482 (-) 186 WP_000391373.1 hypothetical protein -
  HBA75_RS05595 (HBA75_05600) - 1088767..1089348 (+) 582 WP_006924949.1 competence protein ComK -
  HBA75_RS05600 (HBA75_05605) - 1089419..1089718 (-) 300 WP_000655757.1 hypothetical protein -
  HBA75_RS05605 (HBA75_05610) - 1090071..1090664 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  HBA75_RS05610 (HBA75_05615) lepB 1090721..1091284 (+) 564 WP_000751919.1 signal peptidase I -
  HBA75_RS05615 (HBA75_05620) addB 1091401..1094916 (+) 3516 WP_086412620.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  HBA75_RS05620 (HBA75_05625) addA 1094913..1098638 (+) 3726 WP_086412619.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  HBA75_RS05625 (HBA75_05630) - 1098651..1098938 (+) 288 WP_000255728.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  HBA75_RS05630 (HBA75_05635) gerPF 1099071..1099286 (-) 216 WP_001141566.1 spore germination protein GerPF -
  HBA75_RS05635 (HBA75_05640) - 1099330..1099716 (-) 387 WP_000902333.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134053.31 Da        Isoelectric Point: 5.2474

>NTDB_id=430612 HBA75_RS05615 WP_086412620.1 1091401..1094916(+) (addB) [Bacillus thuringiensis strain HER1410]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVANEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNGGVIPAAPMDEGMLSDEEREVLGAAGIEL
GPTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKQIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=430612 HBA75_RS05615 WP_086412620.1 1091401..1094916(+) (addB) [Bacillus thuringiensis strain HER1410]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAACGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGCTATTATACGATGATTTTGAACGTGCTTTAATTGGAAAATACTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACAATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCGCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAGCTTGTTGCGAATGAAAAATATCGTTACCGGGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAGCGCCCGATGTCACACCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGTTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTTCAGAGGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGTTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTATTTTTAGAAGAACTGGATGTTCCGAAAAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAGATGCTTGGTGAGGAGAAA
ATGTCTCTTTCTATGTTTACGGACGTTATGTCGACTGGTCTTGAGGCGCTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGATTATCTGACGTGAAAGCAACGTTTATTATAGGTGTAAATGGGGGTG
TTATTCCAGCAGCACCTATGGACGAAGGTATGCTTTCTGATGAAGAAAGAGAAGTTCTTGGTGCAGCAGGGATTGAATTA
GGGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGCTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGCAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGATGTAAACGATTTATCACGTTCAGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGCAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAATTAAGTACAGCAGTAAGTAGAGATTTATACGGAGATATAATTAAAGGAAGTGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCATACGCTCATTTTGCGCAGCACGGTTTATCGCTTAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCAGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCCATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACTTCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGATTCGTACCAGTTGATTTAGAAGTGCCGTTCGGTATGGGTGGTACAGGATCACTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTCGGTCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTCGTTACTTCGAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TATTGTATTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAGTCTGTTTGTCAATTTGATGAGTCACTGGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(302-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.403

100

0.494