Detailed information NTG0042765

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HJ37_RS10255
• Genome accession: NC_016445
• Coordinates: 2274732..2275748 (-)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae O1 str. 2010EL-1786
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2269732..2280748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HJ37_RS10230 (Vch1786_I2124)	aroK	2270615..2271139 (-)	525	WP_000818616.1	shikimate kinase AroK	-
HJ37_RS10235 (Vch1786_I2125)	pilQ	2271326..2273041 (-)	1716	WP_000788426.1	type IV pilus secretin PilQ	Machinery gene
HJ37_RS10240 (Vch1786_I2126)	pilP	2273067..2273585 (-)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
HJ37_RS10245 (Vch1786_I2127)	pilO	2273575..2274165 (-)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
HJ37_RS10250 (Vch1786_I2128)	pilN	2274158..2274742 (-)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
HJ37_RS10255 (Vch1786_I2129)	pilM	2274732..2275748 (-)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
HJ37_RS10260 (Vch1786_I2130)	-	2275891..2278368 (+)	2478	WP_000668733.1	penicillin-binding protein 1A	-
HJ37_RS10265 (Vch1786_I2131)	oxyR	2278442..2279335 (-)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
HJ37_RS10270 (Vch1786_I2132)	-	2279475..2280206 (+)	732	WP_000495223.1	glutathione peroxidase	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=42765 HJ37_RS10255 WP_000520444.1 2274732..2275748(-) (pilM) [Vibrio cholerae O1 str. 2010EL-1786]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=42765 HJ37_RS10255 WP_000520444.1 2274732..2275748(-) (pilM) [Vibrio cholerae O1 str. 2010EL-1786]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485