Detailed information NTG0427645

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: G7Z36_RS21600
• Genome accession: NZ_CP049752
• Coordinates: 4565497..4566294 (+)
• Length: 265 a.a.
• NCBI ID: WP_004858028.1
• Uniprot ID: -
• Organism: Raoultella ornithinolytica strain WM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4560497..4571294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G7Z36_RS21565 (G7Z36_21565)	gspH	4560650..4561135 (+)	486	WP_406818542.1	type II secretion system minor pseudopilin GspH	-
G7Z36_RS21570 (G7Z36_21570)	gspI	4561132..4561497 (+)	366	WP_054829281.1	type II secretion system minor pseudopilin GspI	-
G7Z36_RS21575 (G7Z36_21575)	gspJ	4561509..4562093 (+)	585	WP_099842791.1	type II secretion system minor pseudopilin GspJ	-
G7Z36_RS21580 (G7Z36_21580)	gspK	4562090..4563076 (+)	987	WP_054829280.1	type II secretion system minor pseudopilin GspK	-
G7Z36_RS21585 (G7Z36_21585)	gspL	4563063..4564265 (+)	1203	WP_015585123.1	type II secretion system protein GspL	-
G7Z36_RS21590 (G7Z36_21590)	gspM	4564258..4564752 (+)	495	WP_088402373.1	type II secretion system protein GspM	-
G7Z36_RS21595 (G7Z36_21595)	-	4564742..4565482 (+)	741	WP_015585125.1	type II secretion system protein N	-
G7Z36_RS21600 (G7Z36_21600)	pilD	4565497..4566294 (+)	798	WP_004858028.1	A24 family peptidase	Machinery gene
G7Z36_RS21605 (G7Z36_21605)	hrpB	4566315..4568744 (-)	2430	WP_088402374.1	ATP-dependent helicase HrpB	-
G7Z36_RS21610 (G7Z36_21610)	thpR	4568817..4569353 (+)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
G7Z36_RS21615 (G7Z36_21615)	sfsA	4569353..4570069 (+)	717	WP_071359157.1	DNA/RNA nuclease SfsA	-
G7Z36_RS21620 (G7Z36_21620)	dksA	4570234..4570689 (+)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29315.95 Da
• Isoelectric Point: 8.4762

>NTDB_id=427645 G7Z36_RS21600 WP_004858028.1 4565497..4566294(+) (pilD) [Raoultella ornithinolytica strain WM1]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLTGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSGLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=427645 G7Z36_RS21600 WP_004858028.1 4565497..4566294(+) (pilD) [Raoultella ornithinolytica strain WM1]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAACTGGCCAGCGGACTGCTCTTTGTGTT
GACCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGCGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCGGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.614	94.717	0.442
pilD	Acinetobacter baumannii D1279779	44.574	97.358	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43