Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   GYO_RS35850 Genome accession   NC_016047
Coordinates   2769167..2771527 (-) Length   786 a.a.
NCBI ID   WP_014114619.1    Uniprot ID   G4NY97
Organism   Bacillus spizizenii TU-B-10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2764167..2776527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYO_RS35830 (GYO_2998) - 2764243..2765796 (+) 1554 WP_041521022.1 SH3 domain-containing protein -
  GYO_RS35835 (GYO_2999) dtd 2765823..2766263 (-) 441 WP_014114616.1 D-aminoacyl-tRNA deacylase -
  GYO_RS35840 (GYO_3000) relA 2766276..2768480 (-) 2205 WP_014114617.1 GTP diphosphokinase -
  GYO_RS35845 (GYO_3001) - 2768649..2769161 (-) 513 WP_014114618.1 adenine phosphoribosyltransferase -
  GYO_RS35850 (GYO_3002) recJ 2769167..2771527 (-) 2361 WP_014114619.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  GYO_RS35855 (GYO_3003) - 2771594..2771926 (-) 333 WP_014114620.1 lipopolysaccharide assembly LapA domain-containing protein -
  GYO_RS35860 (GYO_3004) - 2772004..2772501 (-) 498 WP_014114621.1 cation:proton antiporter regulatory subunit -
  GYO_RS35865 (GYO_3005) secDF 2772657..2774870 (-) 2214 WP_014114622.1 protein translocase subunit SecDF -
  GYO_RS35870 (GYO_3006) comN 2774909..2775205 (-) 297 WP_014114623.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88177.73 Da        Isoelectric Point: 4.9721

>NTDB_id=42603 GYO_RS35850 WP_014114619.1 2769167..2771527(-) (recJ) [Bacillus spizizenii TU-B-10]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EQIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLVITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHVIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELVKLSGGNIGEANEETVGFQIAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLNAEDVPDLRNRLNEIADNTLTEEDFIPVQEVDLVCGVEDITVESITEMNLLSPFGMLN
PKPHVLVENAVLEDVRKIGANKNHVKMTVRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRMWEDTVSVLPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSEEQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKTPERIYFIFLNQEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELARHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGVKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=42603 GYO_RS35850 WP_014114619.1 2769167..2771527(-) (recJ) [Bacillus spizizenii TU-B-10]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTCGAAATGAAGGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAACAGATTATGATATATGGCGATTATGACGCCGATGGAGTTACCAGTACGTCAGTGATGCTTCACACGCTGCAAAAGCT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGCTTTAAAGAAGGATATGGCCCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTCTGGTTATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGGCTGGATGTCATTATTACCGATCACCATGAGCCGGGACCGGAGCTTCCGGATGTCCATGTGATCGTCCACCCGAAGCA
GCCTGGCTGCACCTACCCGTTTAAAGAACTTGCCGGGGTCGGAGTTGCGTTTAAGCTTGCCCACGCTTTATTGGGCGAAC
TCCCGGATGAACTGCTAGACTTGGCGGCAATCGGCACCATTGCCGATCTTGTCCCGTTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGTCGGACGAATCGGCTGGGCTTAAAAGAGCTGGTCAAGCTGTCAGGCGGAAACAT
CGGAGAAGCGAACGAAGAAACAGTCGGGTTCCAGATAGCACCAAGGCTGAATGCCGTCGGACGAATCGAACAGGCGGATC
CCGCTGTTCATTTGCTCATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCTGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGTCTCGATCAAACTGCAATCGT
CGTGGCCAAAGCCGGGTGGAATCCAGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTATCGTCCGGCCA
TTGTGCTTGGCATTGATGAAGAAAAAGGGATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGCTTTAATTTATTCGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGTTGAATGCCGAAGATGT
GCCGGATTTGCGAAACAGGCTGAATGAAATAGCAGACAATACGCTCACGGAAGAGGATTTTATTCCTGTACAGGAAGTCG
ATCTCGTCTGCGGTGTCGAGGACATTACGGTTGAAAGCATCACAGAAATGAACCTGCTGTCCCCATTTGGCATGCTGAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCTGTGCTGGAAGATGTCCGCAAGATCGGCGCTAATAAAAATCATGTGAAAAT
GACGGTCAGAAACGAGTCGTCTCAGCTTGATTGCGTCGGTTTTAATAAAGGTGAGCTCGAAGAAGGCATCGTCCCGGGTT
CAAGAATTTCAATTGTCGGTGAGATGTCTATCAACGAATGGAACAATAGAAAAAAACCGCAGTTAATGATTAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGATGTGGGAAGACACAGTTTCCGTCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACACTTCTTCAGACTGAAGATCTTCGCCGTGAAGTGCACGTCATCA
GCTCTGAAGAACAAGCGAAGGCGTTTGACCTTGATGGTGCATATATTGTATTGCTGGATCCGCCGCCTTCGTTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGACGCCTGAGCGGATATATTTCATTTTTCTCAATCAAGAGGACCACTTTTTATCAAC
ATTTCCCGCAAGAGACCATTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTAAAAAAACACGGAT
CAGAGCTTGCAAGACATAAAGGCTGGTCTGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTAGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTGGTTAGCGGAGTAAAGAAACGCGACCTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHHA1 domain (DHHA1)

(315-437)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NY97

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

97.201

100

0.972

Multiple sequence alignment