Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   G5C61_RS10710 Genome accession   NZ_CP049296
Coordinates   1968531..1970873 (-) Length   780 a.a.
NCBI ID   WP_164796542.1    Uniprot ID   -
Organism   Priestia megaterium strain S188     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1963531..1975873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5C61_RS10685 - 1963924..1964100 (-) 177 WP_013059305.1 hypothetical protein -
  G5C61_RS10690 - 1964267..1965151 (+) 885 WP_241262617.1 N-acetylmuramoyl-L-alanine amidase -
  G5C61_RS10695 dtd 1965142..1965603 (-) 462 WP_164796541.1 D-aminoacyl-tRNA deacylase -
  G5C61_RS10700 - 1965619..1967817 (-) 2199 WP_014458138.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  G5C61_RS10705 - 1968003..1968515 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  G5C61_RS10710 recJ 1968531..1970873 (-) 2343 WP_164796542.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  G5C61_RS10715 secDF 1971014..1973251 (-) 2238 WP_164796543.1 protein translocase subunit SecDF -
  G5C61_RS10720 - 1973389..1973682 (-) 294 WP_025750585.1 post-transcriptional regulator -
  G5C61_RS10725 spoVB 1973747..1975357 (+) 1611 WP_164796544.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87909.53 Da        Isoelectric Point: 4.7708

>NTDB_id=425939 G5C61_RS10710 WP_164796542.1 1968531..1970873(-) (recJ) [Priestia megaterium strain S188]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQNEEQAKACMFAGEQIVLLDLPPSQ
KWLECLFFQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=425939 G5C61_RS10710 WP_164796542.1 1968531..1970873(-) (recJ) [Priestia megaterium strain S188]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTGTTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGTTATCTGCTTTACAGCAGCT
TGGAGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
ACGCGGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAATTA
AATTTTGATTTAATTATTACCGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACAATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGATTATCTCAAGCTCACTAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGCGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATTGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCCGATC
CAGCTGTTCATTTGCTTATGACGACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCGGTTGAATTTGTTGAGAGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAATTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCGAGAAGTATCGAAGGTTTTGACTTATTC
GCGAACCTATCGAAGTGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCGGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCCAAAGTATCGTTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAG
AAGTGGCTTGAATGTTTGTTTTTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGGTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.769

100

0.558