Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   IGW72_RS16465 Genome accession   NZ_CP062219
Coordinates   3578907..3579332 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain JT86     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3573907..3584332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGW72_RS16450 pilX 3574471..3575058 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  IGW72_RS16455 pilY1 3575070..3578561 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  IGW72_RS16460 pilY2 3578563..3578910 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  IGW72_RS16465 comF 3578907..3579332 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  IGW72_RS16470 ispH 3579379..3580323 (-) 945 WP_088002944.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  IGW72_RS16475 fkpB 3580409..3580849 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  IGW72_RS16480 lspA 3580842..3581351 (-) 510 WP_003110420.1 signal peptidase II -
  IGW72_RS16485 ileS 3581344..3584175 (-) 2832 WP_023103515.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=424198 IGW72_RS16465 WP_003094721.1 3578907..3579332(+) (comF) [Pseudomonas aeruginosa strain JT86]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=424198 IGW72_RS16465 WP_003094721.1 3578907..3579332(+) (comF) [Pseudomonas aeruginosa strain JT86]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383