Detailed information NTG0042373

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NH8B_RS17070
• Genome accession: NC_016002
• Coordinates: 3682059..3682925 (-)
• Length: 288 a.a.
• NCBI ID: WP_014088349.1
• Uniprot ID: G2IZF4
• Organism: Pseudogulbenkiania sp. NH8B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3677059..3687925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NH8B_RS17045 (NH8B_3505)	queG	3677552..3678655 (-)	1104	WP_014088344.1	tRNA epoxyqueuosine(34) reductase QueG	-
NH8B_RS17050 (NH8B_3506)	tsaE	3678654..3679145 (+)	492	WP_014088345.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
NH8B_RS17055 (NH8B_3507)	-	3679106..3680338 (+)	1233	WP_014088346.1	N-acetylmuramoyl-L-alanine amidase	-
NH8B_RS20570 (NH8B_3508)	yacG	3680404..3680598 (-)	195	WP_014088347.1	DNA gyrase inhibitor YacG	-
NH8B_RS17060 (NH8B_3509)	zapD	3680607..3681365 (-)	759	WP_040652180.1	cell division protein ZapD	-
NH8B_RS17065 (NH8B_3510)	coaE	3681452..3682072 (-)	621	WP_014088348.1	dephospho-CoA kinase	-
NH8B_RS17070 (NH8B_3511)	pilD	3682059..3682925 (-)	867	WP_014088349.1	A24 family peptidase	Machinery gene
NH8B_RS17075 (NH8B_3512)	pilC	3682925..3684151 (-)	1227	WP_014088350.1	type II secretion system F family protein	Machinery gene
NH8B_RS17080 (NH8B_3513)	pilB	3684175..3685884 (-)	1710	WP_014088351.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NH8B_RS17085 (NH8B_3514)	-	3686134..3687204 (-)	1071	WP_014088352.1	porin	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31438.79 Da
• Isoelectric Point: 7.9672

>NTDB_id=42373 NH8B_RS17070 WP_014088349.1 3682059..3682925(-) (pilD) [Pseudogulbenkiania sp. NH8B]
MMTEMDAVLSANLPLMIGVVSLLGLLVGSFLNVVIHRLPKMLEQEWQLECALLSGQPAQEMPRFNLLTPASHCPSCQAPV
RAWHNIPVLSFLLLRGRCHSCKATIARRYPLVELMCGVMFAALAYRFGWTLDLLGYAALTATLIALAFIDADTQLLPDSL
TLPLLWGGLLFSLLGGRVALDEAVIGAMLGYVSLWVIFWLFKLATGKEGMGYGDFKLLAALGAWLGWKMLPLVILLSSLV
GAVFGIAMILMARLGRGQHLPFGPYLALAGWIALLWGEQIVGWYLGGR

Nucleotide

• Length: 867 bp

>NTDB_id=42373 NH8B_RS17070 WP_014088349.1 3682059..3682925(-) (pilD) [Pseudogulbenkiania sp. NH8B]
ATGATGACGGAGATGGATGCAGTTCTTTCGGCCAACCTTCCGCTGATGATTGGAGTGGTGTCGCTGCTGGGCTTGCTGGT
GGGGAGTTTCCTGAACGTGGTGATTCATCGGCTGCCCAAGATGCTGGAGCAGGAGTGGCAGCTGGAGTGCGCACTGTTGA
GCGGGCAGCCCGCGCAGGAGATGCCGCGTTTCAACCTGCTGACGCCGGCATCGCATTGCCCTTCCTGCCAGGCGCCGGTG
CGTGCCTGGCACAACATCCCAGTGCTCAGTTTCCTGCTGCTGCGCGGCCGTTGCCACAGTTGCAAGGCCACGATTGCGCG
GCGTTATCCGCTGGTCGAGCTGATGTGTGGCGTGATGTTCGCGGCGTTGGCTTACCGCTTCGGCTGGACACTCGACTTGT
TGGGTTACGCTGCCCTGACCGCGACGCTGATTGCACTGGCGTTCATCGATGCCGATACTCAGCTGCTGCCCGATTCGTTG
ACCTTGCCCTTGCTGTGGGGAGGATTGCTGTTCAGCCTGCTGGGCGGGCGGGTGGCGCTGGACGAGGCGGTGATCGGCGC
CATGCTTGGTTACGTCTCGCTGTGGGTGATTTTCTGGTTGTTCAAGCTGGCGACGGGCAAGGAGGGCATGGGGTACGGCG
ACTTCAAGCTGCTGGCGGCACTGGGGGCCTGGCTGGGCTGGAAGATGCTGCCTTTGGTGATCCTGCTGTCGTCGCTGGTC
GGGGCCGTGTTCGGTATTGCCATGATCCTGATGGCGCGACTGGGACGGGGCCAGCATCTGCCGTTCGGCCCCTATCTGGC
GCTGGCTGGCTGGATTGCCCTGCTCTGGGGCGAACAGATCGTTGGGTGGTATCTTGGCGGGCGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

• Type IV leader peptidase family (Peptidase_A24) (139-246)

3D structure

• AlphaFold DB: G2IZF4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.597	93.056	0.517
pilD	Acinetobacter baumannii D1279779	50.347	100	0.503
pilD	Neisseria gonorrhoeae MS11	51.418	97.917	0.503
pilD	Vibrio campbellii strain DS40M4	52.19	95.139	0.497
pilD	Acinetobacter nosocomialis M2	48.958	100	0.49