Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NH8B_RS01620 Genome accession   NC_016002
Coordinates   365144..366226 (-) Length   360 a.a.
NCBI ID   WP_014085753.1    Uniprot ID   G2IW26
Organism   Pseudogulbenkiania sp. NH8B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 360144..371226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH8B_RS01600 (NH8B_0331) - 360386..362167 (+) 1782 WP_014085750.1 PglL family O-oligosaccharyltransferase -
  NH8B_RS01605 (NH8B_0332) rpoH 362358..363221 (-) 864 WP_008955871.1 RNA polymerase sigma factor RpoH -
  NH8B_RS01610 (NH8B_0333) ftsX 363354..364262 (-) 909 WP_014085751.1 permease-like cell division protein FtsX -
  NH8B_RS01615 (NH8B_0334) ftsE 364259..364912 (-) 654 WP_014085752.1 cell division ATP-binding protein FtsE -
  NH8B_RS01620 (NH8B_0335) pilA 365144..366226 (-) 1083 WP_014085753.1 signal recognition particle-docking protein FtsY Machinery gene
  NH8B_RS01625 (NH8B_0336) - 366299..367669 (+) 1371 WP_014085754.1 pitrilysin family protein -
  NH8B_RS01630 (NH8B_0337) - 367659..368978 (+) 1320 WP_014085755.1 pitrilysin family protein -
  NH8B_RS01635 (NH8B_0338) rsmD 369192..369752 (+) 561 WP_014085756.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NH8B_RS01640 (NH8B_0339) - 369854..370105 (+) 252 WP_008955878.1 YfhL family 4Fe-4S dicluster ferredoxin -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 38220.07 Da        Isoelectric Point: 6.8849

>NTDB_id=42361 NH8B_RS01620 WP_014085753.1 365144..366226(-) (pilA) [Pseudogulbenkiania sp. NH8B]
MFSFFKKKSAPPATPASSETPATNTNTTAIAEPHIADAPSTPETAVIAPVVAEVVPPKKPSWTERLKAGLSKTRDKLGKS
LAGLFGGGKIDEDLYDELETVLLTADMGVDATLHLLKDVRERVSLRGLKDAAELKGALKDSLNDLIGPLEQPLDIAGRKP
YVIMMAGVNGAGKTTSIGKLAKYFQSQGKSVLLAAGDTFRAAAREQLIAWGERNNVTVIAQQSGDSAAVCFDAIKAAQAR
GIDIVLADTAGRLPTQLHLMEEIKKVKRVIQKALPEAPHEVMLVLDANIGQNAINQVKVFDEALGLTGLILTKLDGTAKG
GVIAAIAKQNPVPLRFVGVGESIDDLRPFNAKDYVDALFD

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=42361 NH8B_RS01620 WP_014085753.1 365144..366226(-) (pilA) [Pseudogulbenkiania sp. NH8B]
ATGTTTAGTTTCTTCAAGAAGAAGTCTGCGCCGCCGGCCACCCCGGCCAGCAGTGAAACACCCGCCACCAACACCAACAC
CACCGCCATCGCCGAGCCCCACATCGCCGATGCCCCCTCTACCCCCGAAACCGCCGTCATCGCGCCAGTCGTGGCCGAGG
TCGTTCCGCCGAAAAAACCGAGCTGGACCGAGCGGCTCAAGGCCGGCCTGTCGAAAACCCGCGACAAGCTCGGCAAGTCG
CTGGCCGGCCTGTTCGGCGGCGGCAAGATCGACGAGGACCTGTACGACGAACTGGAGACGGTGCTGTTGACCGCCGACAT
GGGCGTCGACGCCACCCTGCACCTGCTCAAGGACGTGCGCGAGCGCGTCTCGCTCCGGGGCCTGAAGGATGCCGCCGAGC
TGAAGGGGGCGCTGAAGGACTCGTTGAACGACTTGATCGGCCCGCTGGAGCAGCCGCTCGACATCGCCGGTCGCAAACCC
TATGTCATCATGATGGCGGGCGTCAACGGCGCTGGCAAGACCACCAGCATCGGCAAGCTGGCCAAGTACTTCCAGTCGCA
GGGCAAGTCGGTGCTGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCGCGCGAGCAGCTGATCGCCTGGGGCGAGCGCA
ACAACGTCACCGTGATCGCGCAGCAGAGCGGCGACTCGGCCGCGGTGTGCTTCGACGCCATCAAGGCGGCGCAGGCGCGC
GGCATCGACATTGTGCTGGCCGACACCGCCGGGCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGATCAAGAAGGTGAA
GCGGGTGATCCAGAAGGCCCTGCCGGAAGCGCCACATGAGGTGATGCTGGTGCTGGACGCCAACATCGGCCAGAACGCCA
TCAACCAAGTGAAGGTGTTCGACGAGGCGCTCGGCCTGACCGGGCTGATCCTGACCAAGCTCGACGGCACCGCCAAGGGC
GGGGTGATCGCCGCCATCGCCAAGCAGAACCCGGTGCCGCTGCGCTTCGTCGGCGTCGGCGAAAGCATCGACGACCTGCG
TCCGTTCAATGCCAAGGACTACGTTGACGCGCTGTTCGACTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(75-142)

SRP54-type protein, GTPase domain (SRP54)

(161-359)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2IW26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

75.578

84.167

0.636

Multiple sequence alignment