Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   IE857_RS02220 Genome accession   NZ_CP061914
Coordinates   460961..462085 (+) Length   374 a.a.
NCBI ID   WP_000228535.1    Uniprot ID   B7LHY7
Organism   Escherichia coli strain SMBL     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 455961..467085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE857_RS02195 (IE857_02195) mscL 456087..456497 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  IE857_RS02200 (IE857_02200) trkA 456627..458003 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  IE857_RS02205 (IE857_02205) rsmB 458025..459314 (-) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  IE857_RS02210 (IE857_02210) fmt 459360..460307 (-) 948 WP_000004454.1 methionyl-tRNA formyltransferase -
  IE857_RS02215 (IE857_02215) def 460322..460831 (-) 510 WP_000114984.1 peptide deformylase -
  IE857_RS02220 (IE857_02220) dprA 460961..462085 (+) 1125 WP_000228535.1 DNA-protecting protein DprA Machinery gene
  IE857_RS02225 (IE857_02225) smg 462057..462530 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  IE857_RS02230 (IE857_02230) yrdD 462559..463101 (+) 543 WP_001129719.1 type I DNA topoisomerase -
  IE857_RS02235 (IE857_02235) tsaC 463106..463678 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  IE857_RS02240 (IE857_02240) aroE 463683..464501 (+) 819 WP_000451211.1 shikimate dehydrogenase -
  IE857_RS02245 (IE857_02245) yrdB 464498..464755 (+) 258 WP_001070603.1 DUF1488 domain-containing protein -
  IE857_RS02250 (IE857_02250) yrdA 464731..465285 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40954.92 Da        Isoelectric Point: 6.3122

>NTDB_id=423580 IE857_RS02220 WP_000228535.1 460961..462085(+) (dprA) [Escherichia coli strain SMBL]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLVTTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=423580 IE857_RS02220 WP_000228535.1 460961..462085(+) (dprA) [Escherichia coli strain SMBL]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGTGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LHY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

44.865

98.93

0.444
  dprA Vibrio cholerae strain A1552

44.865

98.93

0.444
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Glaesserella parasuis strain SC1401

44.838

90.642

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393