Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   IE382_RS17610 Genome accession   NZ_CP061870
Coordinates   3226956..3229313 (-) Length   785 a.a.
NCBI ID   WP_017696683.1    Uniprot ID   -
Organism   Bacillus subtilis strain FX-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3221956..3234313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE382_RS17585 (IE382_17495) etfB 3222398..3223171 (-) 774 WP_017696680.1 electron transfer flavoprotein subunit beta -
  IE382_RS17590 (IE382_17500) fadB 3223186..3223962 (-) 777 WP_017696681.1 enoyl-CoA hydratase -
  IE382_RS17595 (IE382_17505) fadR 3223977..3224561 (-) 585 WP_014477593.1 fatty acid metabolism transcriptional regulator FadR -
  IE382_RS17600 (IE382_17510) lcfA 3224666..3226348 (-) 1683 WP_017696682.1 long-chain-fatty-acid--CoA ligase LcfA -
  IE382_RS17605 (IE382_17515) yshE 3226537..3226941 (-) 405 WP_003237674.1 DUF350 domain-containing protein -
  IE382_RS17610 (IE382_17520) mutS/mutS2 3226956..3229313 (-) 2358 WP_017696683.1 endonuclease MutS2 Machinery gene
  IE382_RS17615 (IE382_17525) polX 3229334..3231046 (-) 1713 WP_003229538.1 DNA polymerase/3'-5' exonuclease PolX -
  IE382_RS17620 (IE382_17530) yshB 3231120..3231653 (-) 534 WP_041351337.1 CvpA family protein -
  IE382_RS17625 (IE382_17535) zapA 3231660..3231917 (-) 258 WP_017696685.1 cell division protein ZapA -
  IE382_RS17630 (IE382_17540) rnhC 3232051..3232989 (+) 939 WP_095843868.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87386.73 Da        Isoelectric Point: 6.1150

>NTDB_id=423541 IE382_RS17610 WP_017696683.1 3226956..3229313(-) (mutS/mutS2) [Bacillus subtilis strain FX-1]
MQQKVLSALEFHKVKEQVIGHAASSLGKEMLLELKPSASIDEIKKQLDEVDEASDIIRLRGQAPFGGLVDIRGALRRAEI
GSVLSPSEFTEISGLLYAVKQMKHFITQMAEDGVDIPLIHQHAEQLITLSDLERDINSCIDDHGEVLDHASETLRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRVLTEKTAEHTEELFLDLQVLQTLDFIFAKARYAKAVKATKPIMNDTGFIRLKKARHPLLPPDQVVAN
DIELGRDFSTIVITGPNTGGKTVTLKTLGLLTLMAQSGLHIPADEGSEAAVFEHVFADIGDEQSIEQSLSTFSSHMVNIV
GILEQVNENSLVLFDELGAGTDPQEGAALAMSILDDVHRTNARVLATTHYPELKAYGYNREGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISKRLGLPDHIIGQAKSEMTAEHNEVDTMIASLEQSKKRAEEELSETESIRKEAEKLHKELQQQIIE
LNSKKDKMLEEAEQQAAEKVKAAMKEAEDIIHELRSIKEEHKSFKDHELINAKKRLEDAMPAFEKSKKPEKPKAQKRDFK
PGDEVKVLTFGQKGTLLEKTGGNEWNVQIGILKMKVKEKDLEFIKSAPEPKKEKIITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTVVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=423541 IE382_RS17610 WP_017696683.1 3226956..3229313(-) (mutS/mutS2) [Bacillus subtilis strain FX-1]
GTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGGCATGCCGCTTCATCGCTCGG
AAAAGAAATGCTTCTCGAGCTTAAGCCTTCTGCTTCTATAGACGAAATCAAAAAACAGCTGGATGAAGTAGACGAAGCTT
CTGACATTATCCGGCTGAGAGGCCAAGCGCCATTTGGCGGCCTTGTAGATATCAGAGGAGCGTTAAGACGGGCGGAAATC
GGCAGCGTTCTCAGTCCTTCTGAATTCACTGAAATCTCAGGCCTGCTTTATGCAGTTAAACAAATGAAACATTTTATCAC
CCAAATGGCTGAAGACGGTGTCGACATTCCGCTGATCCATCAGCATGCTGAACAGCTTATCACGCTGTCCGATTTAGAGC
GGGACATTAATTCCTGCATCGATGATCACGGAGAAGTGCTTGATCATGCATCGGAAACTTTAAGAGGAATCCGCACACAG
CTCAGAACACTCGAATCAAGAGTCAGAGACCGGTTAGAGTCGATGCTGCGTTCCTCTTCCGCATCGAAAATGCTGTCTGA
TACGATTGTTACGATTCGGAATGACCGCTTTGTGATCCCGGTCAAACAGGAGTACAGATCCAGCTATGGAGGAATTGTGC
ACGACACCTCATCCTCTGGTGCGACACTATTCATTGAACCGCAGGCGATTGTAGATATGAACAATTCCCTTCAGCAGGCG
AAAGTGAAAGAAAAGCAAGAAATTGAACGGATTTTGCGTGTGCTGACAGAGAAAACGGCAGAGCATACAGAGGAGCTATT
TCTAGATTTGCAAGTGCTGCAGACACTTGACTTTATTTTTGCAAAAGCTAGATATGCAAAAGCGGTTAAAGCGACAAAAC
CGATTATGAACGACACCGGCTTTATCCGTTTGAAAAAAGCCCGCCATCCATTGCTTCCGCCTGATCAGGTTGTTGCCAAT
GACATCGAGCTTGGCCGCGATTTTTCAACCATTGTCATCACAGGGCCAAACACCGGGGGGAAAACAGTCACCCTTAAAAC
GTTAGGCCTGCTAACCTTAATGGCGCAATCAGGTCTTCATATCCCGGCAGATGAAGGGTCAGAAGCGGCAGTATTTGAGC
ACGTATTCGCTGATATCGGTGATGAACAGTCGATTGAGCAAAGTTTAAGTACGTTCTCATCCCATATGGTGAATATTGTC
GGCATTTTAGAACAGGTCAATGAAAACAGTCTTGTGCTTTTCGATGAACTTGGTGCAGGGACAGATCCGCAGGAGGGGGC
GGCCCTCGCCATGAGCATCTTGGATGACGTGCATCGCACCAATGCAAGAGTGTTAGCTACGACGCATTATCCGGAATTGA
AGGCGTACGGCTATAACAGAGAAGGCGTCATGAATGCCAGTGTTGAATTTGACATCGAAACGCTGTCACCGACCTATAAA
CTTTTAATTGGTGTGCCGGGTCGAAGCAATGCTTTCGAAATTTCAAAACGCCTCGGGCTTCCGGACCATATCATCGGGCA
GGCGAAGTCAGAAATGACGGCCGAGCATAACGAAGTCGATACGATGATTGCGTCGCTGGAACAAAGCAAAAAACGTGCGG
AAGAAGAGCTTTCTGAGACAGAATCAATCAGAAAAGAAGCGGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATCGAG
CTTAACAGCAAAAAAGACAAAATGCTTGAAGAGGCAGAACAGCAGGCTGCTGAAAAAGTAAAAGCGGCAATGAAAGAAGC
CGAGGACATTATTCATGAATTGCGCTCCATAAAAGAAGAACACAAATCCTTCAAGGATCACGAGCTGATTAACGCGAAGA
AACGGTTAGAAGACGCTATGCCGGCTTTTGAAAAGTCCAAGAAACCGGAAAAGCCGAAAGCGCAAAAACGCGACTTTAAG
CCTGGCGACGAGGTGAAAGTCCTCACTTTCGGGCAAAAAGGAACATTGCTCGAAAAAACAGGCGGCAATGAATGGAATGT
TCAAATCGGTATTTTAAAGATGAAAGTAAAAGAAAAAGATCTGGAGTTTATCAAATCAGCTCCGGAGCCAAAAAAAGAAA
AAATCATTACAGCGGTCAAGGGAAAGGACTATCACGTATCGCTTGAACTTGATCTCCGCGGCGAACGCTATGAAAATGCC
CTCAGCCGGGTTGAAAAATACTTGGATGATGCGGTGTTAGCCGGATATCCAAGAGTGTCAATCATCCACGGAAAAGGAAC
CGGCGCTTTAAGAAAAGGCGTACAGGATCTTCTGAAAAACCATCGCAGCGTCAAAAGTTCCCGTTTCGGTGAAGCAGGTG
AGGGAGGATCAGGCGTTACGGTTGTTGAACTAAAATAA

Domains

Predicted by InterProScan.

Smr domain (Smr)

(710-785)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

MutS domain V (MutS_V)

(331-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

99.363

100

0.994