Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   IE752_RS23270 Genome accession   NZ_CP061850
Coordinates   5005653..5006078 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain R31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5000653..5011078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE752_RS23255 (IE752_23255) pilX 5001217..5001804 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  IE752_RS23260 (IE752_23260) pilY1 5001816..5005307 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  IE752_RS23265 (IE752_23265) pilY2 5005309..5005656 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  IE752_RS23270 (IE752_23270) comF 5005653..5006078 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  IE752_RS23275 (IE752_23275) ispH 5006125..5007069 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  IE752_RS23280 (IE752_23280) fkpB 5007155..5007595 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  IE752_RS23285 (IE752_23285) lspA 5007588..5008097 (-) 510 WP_003110420.1 signal peptidase II -
  IE752_RS23290 (IE752_23290) ileS 5008090..5010921 (-) 2832 WP_023103515.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=423077 IE752_RS23270 WP_003094721.1 5005653..5006078(+) (comF) [Pseudomonas aeruginosa strain R31]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=423077 IE752_RS23270 WP_003094721.1 5005653..5006078(+) (comF) [Pseudomonas aeruginosa strain R31]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383