Detailed information NTG0422840

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IFJ78_RS30140
• Genome accession: NZ_CP061779
• Coordinates: 6474462..6475610 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ZBX-P12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6469462..6480610

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IFJ78_RS30115 (IFJ78_30120)	-	6470613..6470909 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
IFJ78_RS30120 (IFJ78_30125)	-	6470906..6471499 (-)	594	WP_003155373.1	YggT family protein	-
IFJ78_RS30125 (IFJ78_30130)	proC	6471510..6472331 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
IFJ78_RS30130 (IFJ78_30135)	-	6472343..6473035 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
IFJ78_RS30135 (IFJ78_30140)	pilT	6473250..6474284 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IFJ78_RS30140 (IFJ78_30145)	pilU	6474462..6475610 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
IFJ78_RS30145 (IFJ78_30150)	czcD	6475618..6476517 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IFJ78_RS30150 (IFJ78_30155)	-	6476672..6477076 (+)	405	WP_003084560.1	NINE protein	-
IFJ78_RS30155 (IFJ78_30160)	-	6477319..6478692 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
IFJ78_RS30160 (IFJ78_30165)	-	6478689..6479873 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=422840 IFJ78_RS30140 WP_003100959.1 6474462..6475610(+) (pilU) [Pseudomonas aeruginosa strain ZBX-P12]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=422840 IFJ78_RS30140 WP_003100959.1 6474462..6475610(+) (pilU) [Pseudomonas aeruginosa strain ZBX-P12]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382