Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   M654_RS17140 Genome accession   NZ_CP048273
Coordinates   3281758..3284118 (-) Length   786 a.a.
NCBI ID   WP_026588081.1    Uniprot ID   A0A6I7FGL7
Organism   Bacillus sp. NSP9.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3276758..3289118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M654_RS17120 (M654_017065) - 3276821..3278392 (+) 1572 WP_026588077.1 SH3 domain-containing protein -
  M654_RS17125 (M654_017070) dtd 3278418..3278861 (-) 444 WP_026588078.1 D-aminoacyl-tRNA deacylase -
  M654_RS17130 (M654_017075) - 3278874..3281075 (-) 2202 WP_026588079.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  M654_RS17135 (M654_017080) - 3281240..3281752 (-) 513 WP_026588080.1 adenine phosphoribosyltransferase -
  M654_RS17140 (M654_017085) recJ 3281758..3284118 (-) 2361 WP_026588081.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  M654_RS17145 (M654_017090) - 3284172..3284480 (-) 309 WP_026588082.1 lipopolysaccharide assembly LapA domain-containing protein -
  M654_RS17150 (M654_017095) secDF 3284632..3286857 (-) 2226 WP_026588083.1 protein translocase subunit SecDF -
  M654_RS17155 (M654_017100) - 3286896..3287192 (-) 297 WP_026588084.1 post-transcriptional regulator -
  M654_RS17160 (M654_017105) spoVB 3287309..3288862 (+) 1554 WP_026588085.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88324.03 Da        Isoelectric Point: 5.1460

>NTDB_id=420470 M654_RS17140 WP_026588081.1 3281758..3284118(-) (recJ) [Bacillus sp. NSP9.1]
MLASKMRWDVKTPDKEKVELLSEQLGITPLAASLLVMRGYDTADKAASFLYGKDAVFYDPYLLKDMEKAVQRIKQAISEN
EKIMIYGDYDADGVTSTTVLLQILKKYSAQADFYIPDRFKEGYGPNEAAFRHIKEQGYSLIVTVDTGIAGVHEAEVAKEL
GIDLIITDHHEPGPVLPDVYAIIHPKQPGCSYPFKELAGVGVAFKLAHALNGDIPEDLLDLCAIGTIADLVPLHDENRHI
AKLGMARFRKTNRPGLKALAKIAGAEIQSADEETIGFQIAPRINAVGRIVQADPAVYLLNTEDDTEAGELAEEINALNKE
RQKIVNQITEEAVQMIEDEGLDQSVIVCARAGWNPGVVGIVASKLVERFYRPAIVLGIDEEKGIAKGSARSIKGFDLFQN
LSECRDILPHFGGHPMAAGMTLSQEDVPLLRERINRQAEAVLTAEDFIPVQEVDLCCRLEDITVDSIAEMNKLAPFGTGN
PKPFVLVSDAKIDEARKIGANQNHLKLVLADEGTKLDCVGFYKGDIEEKIVSGANISIVGELSINEWNNRKKPQLMIKDA
AVNEWQLFDFRGKRSWKEKILKLDPAKRIFLCFREETKTTFAGTELADELIFISGEEEAKRLDLHNRYAVFIDTPQSLER
FDHAVKGKKLERIYLIFHQSDEHFFSTFPTRDHFKWYYAFLLKRGSFDLAKYAGELARHKGWTKDTIDFMTKVFFELGFV
TIENGVLSVKFDAAKRDLTESDTYREKQRLIELEQKLVYSSADELKQWLNKLMGESAEAYESMRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=420470 M654_RS17140 WP_026588081.1 3281758..3284118(-) (recJ) [Bacillus sp. NSP9.1]
ATGTTAGCGTCAAAAATGCGTTGGGATGTGAAAACACCCGACAAAGAAAAGGTGGAGCTGCTCTCCGAACAGTTGGGTAT
CACTCCCCTTGCCGCCTCGTTGCTTGTCATGAGAGGCTACGATACAGCAGACAAAGCAGCTTCATTTTTGTACGGCAAAG
ATGCTGTCTTTTATGATCCTTATCTGCTTAAAGATATGGAGAAAGCTGTACAGCGAATCAAACAGGCCATTTCAGAAAAT
GAAAAGATTATGATTTATGGAGATTATGATGCGGACGGTGTGACAAGCACGACTGTCCTCCTGCAGATCTTAAAAAAATA
TTCAGCTCAAGCCGATTTTTATATACCTGACCGCTTTAAAGAAGGCTACGGCCCGAATGAAGCCGCATTCCGGCATATAA
AGGAACAGGGCTATTCACTGATTGTCACGGTGGATACGGGGATTGCCGGTGTTCACGAAGCAGAGGTCGCAAAAGAGCTC
GGCATCGACTTGATTATTACCGATCACCATGAGCCTGGTCCGGTTTTGCCTGACGTCTATGCGATCATTCATCCGAAGCA
GCCCGGATGCAGCTATCCTTTCAAAGAGCTGGCCGGCGTCGGGGTCGCATTTAAGCTTGCTCATGCTTTGAACGGCGACA
TTCCTGAGGACCTTCTTGATCTTTGTGCGATCGGAACGATTGCCGATCTTGTGCCGCTTCATGATGAAAACAGGCACATC
GCCAAGCTCGGGATGGCCCGGTTTAGAAAGACGAACCGCCCCGGCTTAAAAGCGCTCGCGAAGATTGCGGGAGCCGAGAT
TCAAAGCGCGGATGAAGAGACGATCGGGTTTCAAATTGCTCCGCGAATCAATGCCGTCGGCCGAATCGTTCAGGCTGATC
CGGCTGTTTATCTTTTAAACACAGAAGATGACACAGAAGCCGGGGAGCTGGCTGAAGAAATTAACGCACTGAACAAAGAA
CGGCAAAAAATCGTCAACCAGATCACAGAAGAAGCGGTCCAAATGATAGAGGACGAGGGTCTGGACCAGTCTGTCATCGT
CTGTGCGCGGGCCGGCTGGAATCCGGGCGTTGTCGGGATCGTCGCCTCCAAGCTGGTCGAACGGTTTTACCGGCCGGCCA
TCGTTCTCGGAATTGATGAAGAAAAAGGGATTGCCAAAGGCTCTGCGCGCAGCATTAAAGGCTTTGACCTGTTTCAAAAC
CTTTCGGAATGCCGGGATATCCTTCCCCATTTTGGCGGCCACCCGATGGCGGCCGGAATGACGCTCAGCCAGGAAGATGT
TCCGCTATTAAGAGAACGAATCAACCGCCAGGCTGAAGCTGTTTTAACAGCGGAAGACTTCATTCCCGTCCAGGAAGTCG
ACCTTTGCTGCCGATTGGAAGATATTACGGTGGACAGCATCGCAGAAATGAACAAGCTGGCTCCGTTCGGCACGGGAAAC
CCGAAGCCGTTCGTCCTTGTCAGCGACGCCAAAATTGATGAGGCGAGAAAAATCGGCGCCAATCAGAATCACCTAAAGCT
CGTGTTGGCAGACGAAGGGACAAAGCTTGATTGTGTCGGATTCTATAAAGGCGATATCGAAGAAAAAATCGTATCCGGCG
CAAATATTTCCATTGTCGGCGAATTGTCGATCAATGAATGGAATAACAGAAAAAAGCCGCAGCTCATGATCAAGGATGCG
GCCGTAAACGAATGGCAGCTGTTTGACTTCCGGGGAAAGCGTTCATGGAAAGAAAAAATCTTAAAGCTTGATCCGGCAAA
ACGGATATTCCTTTGTTTCCGGGAAGAAACGAAAACGACATTTGCGGGAACTGAGCTCGCAGATGAGCTGATCTTCATCA
GCGGGGAAGAGGAGGCAAAGCGTCTCGATCTACATAACCGTTATGCGGTTTTCATTGACACGCCCCAGTCGCTTGAGAGG
TTCGACCATGCTGTAAAAGGCAAAAAGCTTGAGCGGATTTATCTGATTTTTCATCAGTCTGACGAGCATTTTTTCTCGAC
GTTTCCAACGAGGGATCATTTCAAATGGTACTACGCTTTCCTTCTCAAACGAGGCTCGTTTGACTTAGCAAAATACGCCG
GAGAGCTTGCCAGACATAAAGGCTGGACAAAAGACACAATTGATTTTATGACAAAGGTGTTTTTTGAGCTCGGTTTTGTT
ACAATAGAGAATGGTGTTTTATCTGTTAAATTCGATGCCGCGAAGCGGGATTTAACAGAATCTGACACTTACCGTGAAAA
ACAGCGCCTGATTGAATTGGAGCAAAAGCTCGTCTATTCTTCGGCGGACGAGCTCAAACAGTGGCTGAATAAGCTGATGG
GCGAAAGCGCTGAAGCTTATGAAAGTATGAGGAGGACATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-436)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I7FGL7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

68.83

100

0.688

Multiple sequence alignment