Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   M654_RS15830 Genome accession   NZ_CP048273
Coordinates   3032904..3033941 (-) Length   345 a.a.
NCBI ID   WP_026587853.1    Uniprot ID   A0A6I7FEV0
Organism   Bacillus sp. NSP9.1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3027904..3038941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M654_RS15780 (M654_015730) - 3027943..3028737 (-) 795 WP_026587843.1 TasA family protein -
  M654_RS15785 (M654_015735) - 3028806..3029390 (-) 585 WP_026587844.1 signal peptidase I -
  M654_RS15790 (M654_015740) tapA 3029387..3030115 (-) 729 WP_026587845.1 amyloid fiber anchoring/assembly protein TapA -
  M654_RS15795 (M654_015745) - 3030385..3030702 (+) 318 WP_026587846.1 YqzG/YhdC family protein -
  M654_RS15800 (M654_015750) - 3030789..3030974 (-) 186 WP_026587847.1 YqzE family protein -
  M654_RS15805 (M654_015755) comGG 3031046..3031411 (-) 366 WP_026587848.1 competence type IV pilus minor pilin ComGG -
  M654_RS24145 (M654_015760) comGF 3031425..3031913 (-) 489 WP_077736493.1 competence type IV pilus minor pilin ComGF -
  M654_RS15815 (M654_015765) comGE 3031822..3032169 (-) 348 WP_026587850.1 competence type IV pilus minor pilin ComGE -
  M654_RS15820 (M654_015770) comGD 3032153..3032596 (-) 444 WP_236251143.1 competence type IV pilus minor pilin ComGD -
  M654_RS15825 (M654_015775) comGC 3032596..3032889 (-) 294 WP_026587852.1 competence type IV pilus major pilin ComGC Machinery gene
  M654_RS15830 (M654_015780) comGB 3032904..3033941 (-) 1038 WP_026587853.1 competence type IV pilus assembly protein ComGB Machinery gene
  M654_RS15835 (M654_015785) comGA 3033928..3034995 (-) 1068 WP_026587854.1 competence type IV pilus ATPase ComGA Machinery gene
  M654_RS15840 (M654_015790) - 3035165..3036016 (-) 852 WP_026587855.1 STAS domain-containing protein -
  M654_RS15850 (M654_015800) - 3036267..3036644 (-) 378 WP_035428189.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  M654_RS15855 (M654_015805) - 3036853..3037098 (+) 246 WP_026587856.1 DUF2626 domain-containing protein -
  M654_RS15860 (M654_015810) - 3037140..3037778 (-) 639 WP_026587857.1 MBL fold metallo-hydrolase -
  M654_RS15865 (M654_015815) - 3037937..3038110 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  M654_RS15870 (M654_015820) - 3038186..3038497 (-) 312 WP_026587858.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 40007.67 Da        Isoelectric Point: 9.7490

>NTDB_id=420462 M654_RS15830 WP_026587853.1 3032904..3033941(-) (comGB) [Bacillus sp. NSP9.1]
MSRNKGKWPVREQAELLEKLGEMIVNGYTLLDALSMLALQLNKHQRADISFGIRKLTEGHPVFQVLNMMSFHQDAVSIVY
FAEQHGNLPFAFCQSGELLHRKIGQAEKIKKAAKYPLFLILTVCLISYILKSAILPQFSAIYDSMNIERTFLTSFIFLFF
DSFTWLFILMLLLVAVLAVYYVCRFRHKPPEARMLLFIKIPLLGRILKLFNSYFFSLQLSNLLKSGLSIYESLKAFESQP
FLPFYQHEAKCLIKRLKQGEAIDEMIAGRPYYEKDLSKAIVHGQLNGHLHRELYSYSQFLLDRLEKKAAKWAGMIQPMIY
GLTALMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=420462 M654_RS15830 WP_026587853.1 3032904..3033941(-) (comGB) [Bacillus sp. NSP9.1]
ATGAGTCGGAATAAAGGCAAATGGCCTGTGCGGGAACAGGCGGAGCTTCTTGAAAAGCTCGGCGAGATGATAGTAAACGG
CTATACGCTGCTGGACGCTTTAAGCATGCTGGCGCTGCAATTGAACAAACATCAAAGAGCGGACATCTCGTTTGGAATAC
GGAAGCTTACTGAAGGACACCCTGTTTTTCAAGTTTTGAATATGATGTCATTTCATCAAGACGCCGTCAGCATTGTCTAT
TTTGCCGAGCAGCATGGGAATTTGCCCTTCGCTTTCTGCCAAAGCGGAGAGCTGCTTCACCGTAAAATCGGCCAGGCCGA
AAAAATCAAGAAAGCCGCAAAGTATCCTTTATTCTTAATTTTGACGGTCTGTCTCATCTCTTATATTTTAAAATCGGCCA
TTCTGCCGCAATTCTCAGCGATCTACGATTCGATGAATATCGAGAGAACTTTTTTGACTTCCTTCATATTTTTGTTTTTT
GACAGTTTCACATGGCTGTTCATCCTCATGCTGCTGTTGGTCGCAGTTTTAGCTGTGTACTATGTTTGCCGCTTCCGGCA
TAAGCCTCCTGAAGCCCGAATGCTTCTTTTCATCAAAATTCCTCTGCTTGGCAGAATCCTCAAATTATTTAACAGCTATT
TTTTTTCACTTCAGCTGAGCAACCTTTTAAAATCCGGTTTGTCTATATACGAAAGTCTAAAAGCATTTGAGAGCCAGCCT
TTTTTGCCGTTTTATCAGCACGAGGCCAAGTGTTTGATCAAGAGGCTGAAACAAGGGGAAGCGATCGATGAAATGATAGC
CGGACGCCCCTATTATGAAAAAGATTTGTCCAAAGCGATTGTTCACGGCCAGTTAAACGGTCATCTTCACAGAGAACTAT
ATTCGTACAGCCAATTTCTGCTCGATCGTCTCGAAAAGAAAGCGGCAAAGTGGGCAGGCATGATCCAGCCAATGATTTAC
GGACTGACCGCGCTGATGATACTCATTCTTTATCTGTCTATGCTTTTGCCAATGTATCAAATGATGAATCAGCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I7FEV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.585

93.623

0.539

Multiple sequence alignment