Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   IBG29_RS08985 Genome accession   NZ_CP061073
Coordinates   1933606..1934070 (-) Length   154 a.a.
NCBI ID   WP_058169120.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PAD8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1928606..1939070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IBG29_RS08970 (IBG29_25080) nadC 1931128..1931976 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IBG29_RS08980 (IBG29_25070) - 1932157..1933248 (-) 1092 WP_242790445.1 O-antigen ligase family protein -
  IBG29_RS08985 (IBG29_25065) pilA/pilAI 1933606..1934070 (-) 465 WP_058169120.1 pilin Machinery gene
  IBG29_RS08990 (IBG29_25060) pilB 1934301..1936001 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IBG29_RS08995 (IBG29_25055) pilC 1936005..1937222 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  IBG29_RS09000 (IBG29_25050) pilD 1937223..1938095 (+) 873 WP_016264267.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  IBG29_RS09005 (IBG29_25045) coaE 1938092..1938703 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  IBG29_RS09010 (IBG29_25040) yacG 1938700..1938900 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16315.76 Da        Isoelectric Point: 9.4242

>NTDB_id=419962 IBG29_RS08985 WP_058169120.1 1933606..1934070(-) (pilA/pilAI) [Pseudomonas aeruginosa strain PAD8]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSTKTPNNTQYDIGFTE
STLLDLKGKEQIKVTDNKNGTVQLVATLGGSSGSAIKGAVITVSRDAQGVWSCNITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=419962 IBG29_RS08985 WP_058169120.1 1933606..1934070(-) (pilA/pilAI) [Pseudomonas aeruginosa strain PAD8]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCTCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCACCAAGACTCCTAACAATACCCAGTATGACATTGGCTTTACTGAA
TCTACTTTGCTGGATCTCAAGGGTAAAGAACAGATTAAAGTTACGGATAATAAAAATGGTACCGTGCAGCTAGTTGCTAC
TCTGGGTGGTTCTTCTGGTTCCGCCATCAAAGGAGCTGTCATCACTGTTTCGCGTGACGCTCAAGGAGTCTGGAGCTGCA
ATATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.231

100

0.448
  pilA Pseudomonas aeruginosa PAK

43.312

100

0.442
  pilA Acinetobacter baumannii strain A118

44.079

98.701

0.435
  pilA Ralstonia pseudosolanacearum GMI1000

35.955

100

0.416
  comP Acinetobacter baylyi ADP1

39.024

100

0.416
  pilA Vibrio parahaemolyticus RIMD 2210633

39.333

97.403

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.419

100

0.377
  pilE Neisseria gonorrhoeae MS11

42.537

87.013

0.37
  pilA Vibrio cholerae C6706

36.129

100

0.364
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.129

100

0.364
  pilA Vibrio cholerae strain A1552

36.129

100

0.364

Multiple sequence alignment