Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   H9445_RS18695 Genome accession   NZ_CP060709
Coordinates   3778631..3779395 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain ST18     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3773631..3784395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9445_RS18675 (H9445_18675) acpT 3774585..3775172 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  H9445_RS18680 (H9445_18680) nikA 3775283..3776857 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  H9445_RS18685 (H9445_18685) nikB 3776857..3777801 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  H9445_RS18690 (H9445_18690) nikC 3777798..3778631 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  H9445_RS18695 (H9445_18695) amiE 3778631..3779395 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  H9445_RS18700 (H9445_18700) nikE 3779392..3780198 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  H9445_RS18705 (H9445_18705) nikR 3780204..3780605 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=416835 H9445_RS18695 WP_001136229.1 3778631..3779395(+) (amiE) [Escherichia coli strain ST18]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=416835 H9445_RS18695 WP_001136229.1 3778631..3779395(+) (amiE) [Escherichia coli strain ST18]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment